Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titanium medical devices
Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titanium medical devices
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DOI：
10.1016/j.ijantimicag.2008.06.030
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发表时间：
2008-12-01
影响因子：
10.8
通讯作者：
Watanabe, Akira
中科院分区：
文献类型：
--
作者：
Fujimura, Shigeru;Sato, Tetsuro;Watanabe, Akira
在本研究中，我们研究了克拉霉素（CLA）联合头孢唑啉（CFZ）或万古霉素（VCM）对钛器械上形成的金黄色葡萄球菌生物膜的体外疗效，以确认根除治疗对器械相关感染的疗效。采用液相色谱/串联质谱法对10例骨科患者骨周围肌肉组织中CLA的分布进行了研究。生物膜的形成和根除S.分别通过扫描电子显微镜和使用双染色染料监测金黄色葡萄球菌。虽然S.单独使用CLA、CFZ或VCM不能清除金黄色葡萄球菌生物膜，CLA与CFZ或VCM联合使用可破坏金黄色葡萄球菌生物膜; 72小时后明显观察到金黄色葡萄球菌根除。这项体外研究表明，CLA与CFZ或VCM联合处理可破坏医疗器械上形成的葡萄球菌生物膜，并根除S。金黄色。(C)2008年爱思唯尔B。和国际化疗学会。All rights reserved.
In this study, we investigated the in vitro efficacy of clarithromycin (CLA) combined with cefazolin (CFZ) or vancomycin ( VCM) against Staphylococcus aureus biofilms formed on titanium devices in order to confirm the efficacy of eradication therapies against device-related infection. The distribution of CLA in muscle tissue surrounding bone was also investigated by liquid chromatography/tandem mass spectrometry in 10 orthopaedic patients. Biofilm formation and eradication of S. aureus were monitored by scanning electron microscopy and using double-staining dyes, respectively. Although S. aureus biofilms were not eradicated by CLA, CFZ or VCM alone, CLA combined with CFZ or VCM destroyed biofilms, and S. aureus eradication was clearly observed 72 h later. This in vitro study showed that treatment with CLA plus CFZ or VCM destroyed staphylococcal biofilms formed on medical devices and eradicated S. aureus. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.