SlHair2 Regulates the Initiation and Elongation of Type I Trichomes on Tomato Leaves and Stems
SlHair2 Regulates the Initiation and Elongation of Type I Trichomes on Tomato Leaves and Stems
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DOI：
10.1093/pcp/pcab090
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发表时间：
2021-06-22
影响因子：
4.9
通讯作者：
Kang, Jin-Ho
中科院分区：
文献类型：
--
作者：
Chun, Jae-In;Kim, Seong-Min;Kang, Jin-Ho
毛状体是毛发状结构，对非生物和生物应激反应至关重要。研究发现，编码C2H2锌指蛋白的番茄毛（Tomato Hair， H）调控茎上的多细胞毛状体。在这里，我们对其最接近的同源物Solyc10g078990（以下简称Hair2， H2）进行了表征，以研究它是否参与了毛状体的发育。H2基因在叶片中高表达，其蛋白包含一个C2H2结构域，定位于细胞核。与野生型相比，敲除h2植株叶片和茎上I型毛状体的数量和长度减少，而过表达则增加了它们的数量和长度。利用酵母双杂交（Y2H）对不同截断形式的H2进行了自激活试验，结果表明H2作为转录调节因子或共激活因子，其n端区域对自激活很重要。Y2H和pull-down分析表明，H2与Woolly （Wo）相互作用，调控番茄I型毛状体的发育。荧光素酶互补成像分析证实它们有直接的相互作用，这意味着H2和Wo共同调节毛状体的发育。这些结果表明，H2在番茄I型毛状体的起始和伸长中起作用。
Trichomes are hair-like structures that are essential for abiotic and biotic stress responses. Tomato Hair (H), encoding a C2H2 zinc finger protein, was found to regulate the multicellular trichomes on stems. Here, we characterized Solyc10g078990 (hereafter Hair2, H2), its closest homolog, to examine whether it was involved in trichome development. The H2 gene was highly expressed in the leaves, and its protein contained a single C2H2 domain and was localized to the nucleus. The number and length of type I trichomes on the leaves and stems of knock-out h2 plants were reduced when compared to the wild-type, while overexpression increased their number and length. An auto-activation test with various truncated forms of H2 using yeast two-hybrid (Y2H) suggested that H2 acts as a transcriptional regulator or co-activator and that its N-terminal region is important for auto-activation. Y2H and pull-down analyses showed that H2 interacts with Woolly (Wo), which regulates the development of type I trichomes in tomato. Luciferase complementation imaging assays confirmed that they had direct interactions, implying that H2 and Wo function together to regulate the development of trichomes. These results suggest that H2 has a role in the initiation and elongation of type I trichomes in tomato.