Characterization of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm
Characterization of a modified rotating disk reactor for the cultivation of Staphylococcus epidermidis biofilm
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DOI：
10.1111/j.1365-2672.2010.04842.x
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发表时间：
2010-12-01
影响因子：
4
通讯作者：
Casey, E.
中科院分区：
文献类型：
--
作者：
Cotter, J. J.;O'Gara, J. P.;Casey, E.
目的：本研究的目的是开发一种在溶解氧、流体剪切等严格限定条件下培养生物膜的系统，并评估该方法是否适用于生物膜样品中呼吸活动分层的检测。方法：该系统是市售实验室生物膜反应器的改进版本，并结合了许多功能，如提供规定水平的溶解氧，恒定平均剪切，增强气液传质，无菌操作以及在连续培养期间或之后去除生物膜进行非原位分析的能力。结论：该系统被证明是有效的表征溶解氧对表皮葡萄球菌纯培养的影响。该系统的多功能性提供了在定义的、受控的条件下培养纯培养生物膜的潜力，并促进了一系列可以在非原位进行的分析。研究的意义和影响：在培养过程中提供严格的环境条件调节和增强氧气向生物膜转移的能力是重要的，首先因为已知氧气可以调节几种微生物的生物膜发育，其次因为许多传统的生物膜培养系统可能无法为生物膜提供足够的氧气供应。
Aims: The purpose of this study was to develop a system that would allow biofilms to be cultivated under strictly defined conditions in terms of dissolved oxygen, fluid shear and to assess whether the method was suitable for the detection of respiratory activity stratification in biofilm samples.Methods: The system is a modified version a commercially available laboratory biofilm reactor and incorporates a number of features such as the provision of defined levels of dissolved oxygen, constant average shear, enhanced gas-liquid mass transfer, aseptic operation and the ability to remove biofilm for ex situ analysis during or after continuous cultivation.Conclusions: The system was shown to be effective for the characterization of the effects of dissolved oxygen on a pure culture of Staphylococcus epidermidis. The versatility of the system offers the potential for cultivating pure culture biofilm in defined, controlled conditions and facilitates a range of analyses that can be performed ex situ.Significance and Impact of the Study: The ability to provide strict regulation of environmental conditions and enhanced transfer of oxygen to the biofilm during cultivation are important, first because oxygen is known to regulate biofilm development in several micro-organisms and second because many conventional biofilm cultivation systems may not provide adequate oxygen supply to the biofilm.