F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons.
F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons.
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DOI：
10.1242/dev.126.16.3637
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发表时间：
1999-08
期刊：
影响因子：
4.6
通讯作者：
Y. Feinstein;V. Borrell;C. García;T. Burstyn-Cohen;V. Tzarfaty;A. Frumkin;A. Nose;H. Okamoto;S. Higashijima;E. Soriano;A. Klar
中科院分区：
文献类型：
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作者：
Y. Feinstein;V. Borrell;C. García;T. Burstyn-Cohen;V. Tzarfaty;A. Frumkin;A. Nose;H. Okamoto;S. Higashijima;E. Soriano;A. Klar
细胞外基质(ECM)蛋白在早期皮质发育中发挥着重要作用，特别是在神经连接的形成和控制中枢神经系统的细胞结构方面。F-响应素和Mindin是一类基质附着的黏附分子，它们具有相似的结构和重叠的表达结构域。这两种蛋白的基因都含有C末端的凝血酶反应蛋白I型重复序列(S)和fs1-fs2(Spaindin)结构域。脊椎动物的F-Spinin和斑马鱼的思维都在胚胎的底板上表达。在本研究中，我们克隆了大鼠Mindin的同源物，并与F-pondin一起研究了其在发育中的啮齿动物脑中的表达和活性。这两个基因在发育中的海马体中大量表达。体外研究表明，F-Spinin和Mindin都能促进海马神经元的黏附和突起生长。我们还证明了这两种蛋白与一个可能的受体(S)结合，该受体在海马神经元和感觉神经元上都有表达。
Extracellular matrix (ECM) proteins play an important role in early cortical development, specifically in the formation of neural connections and in controlling the cyto-architecture of the central nervous system. F-spondin and Mindin are a family of matrix-attached adhesion molecules that share structural similarities and overlapping domains of expression. Genes for both proteins contain a thrombospondin type I repeat(s) at the C terminus and an FS1-FS2 (spondin) domain. Both the vertebrate F-spondin and the zebrafish mindins are expressed on the embryonic floor plate. In the current study we have cloned the rat homologue of mindin and studied its expression and activity together with F-spondin in the developing rodent brain. The two genes are abundantly expressed in the developing hippocampus. In vitro studies indicate that both F-spondin and Mindin promote adhesion and outgrowth of hippocampal embryonic neurons. We have also demonstrated that the two proteins bind to a putative receptor(s) expressed on both hippocampal and sensory neurons.