The occurrence of nitric oxide synthase-containing axonal baskets surrounding large neurons in rat dorsal root ganglia after sciatic nerve ligation.
The occurrence of nitric oxide synthase-containing axonal baskets surrounding large neurons in rat dorsal root ganglia after sciatic nerve ligation.
复制标题
坐骨神经结扎后大鼠背根神经节大神经元周围含有一氧化氮合酶的轴突篮的出现。
DOI：
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复制
发表时间：
2005
影响因子：
--
通讯作者：
M. Kawabuchi
中科院分区：
文献类型：
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作者：
Wenting Liu;K. Hirata;M. Kawabuchi
为探讨外周神经切断后一氧化氮（NO）在初级感觉神经元中的可能作用，采用免疫组织化学方法对大鼠坐骨神经结扎后L5背根神经节NO合成酶（NOS）进行了研究。结果与27 kDa热休克蛋白（HSP 27），神经保护分子的表达进行了比较。在完整的动物中，NOS免疫反应阳性神经元约占所有背根神经节（DRG）神经元的2%，而HSP 27免疫反应阳性神经元约占14%。坐骨神经结扎后，这两个神经元的数量和免疫反应性增加，达到最大值，在2周时，NOS和HSP 27免疫反应阳性神经元代表约33%和66%，分别。NOS免疫反应阳性神经元，然后保持不变，直到7周，虽然HSP 27免疫反应阳性神经元略有下降。增加的NOS免疫反应性神经元优先是小的（100-500 microm（2））并且与HSP 27共表达（约87%）。另一方面，在坐骨神经近端，许多NOS免疫反应纤维的再生轮廓出现短暂（2-4周）。在更高的放大倍数，轴突芽从NOS免疫反应小DRG神经元被发现形成一个篮子状结构（或篮子）主要是周围的NOS阴性大神经元的细胞体。用荧光示踪剂逆行标记显示，两个神经元都向坐骨神经发送外周轴突侧支。这种独特结构的出现在坐骨神经中的NOS免疫反应性再生纤维耗尽后（7-9周）最为突出。结果提示，NO不仅参与了周围神经损伤后的轴突再生，而且参与了两类DRG神经元的重连。
To clarify the possible role of nitric oxide (NO) induced in primary sensory neurons after peripheral axotomy, NO synthase (NOS) immunohistochemistry was carried out on rat L5 dorsal root ganglia after sciatic nerve ligation. The results were compared with the expression of 27-kDa heat shock protein (HSP27), a neuroprotective molecule. In intact animals, NOS-immunoreactive neurons represented about 2% of all dorsal root ganglion (DRG) neurons, whereas HSP27-immunoreactive neurons comprised about 14%. After sciatic nerve ligation, both neurons increased, in number and immunoreactivity, reaching a maximum at 2 weeks, when NOS- and HSP27-immunoreactive neurons represented about 33 and 66%, respectively. NOS-immunoreactive neurons then remained unchanged until 7 weeks although HSP27-immunoreactive neurons showed a slight decline. The increased NOS-immunoreactive neurons were preferentially small (100-500 microm(2)) and coexpressed with HSP27 (about 87%). On the other hand, in the proximal stump of sciatic nerves, numerous NOS-immunoreactive fibers with a regenerative profile appeared transiently (2-4 weeks). At higher magnification, an axonal sprout from the NOS-immunoreactive small DRG neurons was found to form a basket-like structure (or basket) mostly around the cell body of NOS-negative large neurons. Retrograde labeling with a fluorescent tracer showed that both neurons sent peripheral axon collaterals to the sciatic nerve. The appearance of this unique structure was most prominent after depletion of the NOS-immunoreactive regenerating fibers in the sciatic nerve (at 7-9 weeks). The findings suggest that NO might be involved in not only axonal regeneration but also the rewiring of two classes of DRG neurons after peripheral nerve injury.
DOI：
10.1073/pnas.87.2.682
发表时间：
1990-01-01
影响因子：
11.1
作者：
BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH
Alpha B-晶状体蛋白和 27-kd 热休克蛋白受中枢神经系统应激条件的调节，并在 Rosenthal 纤维中积累。
DOI：
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发表时间：
1993
期刊：
The American journal of pathology
影响因子：
--
作者：
Iwaki,T;Iwaki,A;Tateishi,J;Sakaki,Y;Goldman,JE
通讯作者：
Goldman,JE