THE DETECTION AND CHARACTERIZATION OF BACTERIA-SIZED PROTISTS IN PROTIST-FREE FILTRATES AND THEIR POTENTIAL IMPACT ON EXPERIMENTAL MARINE ECOLOGY
THE DETECTION AND CHARACTERIZATION OF BACTERIA-SIZED PROTISTS IN PROTIST-FREE FILTRATES AND THEIR POTENTIAL IMPACT ON EXPERIMENTAL MARINE ECOLOGY
复制标题
DOI：
10.1007/bf02016812
复制
发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
SIEBURTH, JM
中科院分区：
文献类型：
--
作者：
CYNAR, FJ;ESTEP, KW;SIEBURTH, JM
孔径为0.6-1.0 μ m的核孔过滤器已被用于制备“无原生生物”水，用于微生物生态学的许多研究。最近的一项研究对这一过程提出了质疑，该研究声称滤液中很大一部分细菌损失可能是由于未表征的捕食者通过0.6 μ m过滤器造成的。我们不能使用相差、落射荧光或透射电子显微镜直接观察0.6 μ m的原生生物。然而，使用稻粒富集和最可能数的培养技术，我们能够观察和定量几种食菌纳米鞭毛虫，它们不仅在0.6 μ m海水中发育，而且在0.4 μ m海水中发育。捕食性纳米鞭毛虫通过细菌大小的孔隙的能力对细菌生产和化学循环的研究提出了质疑，这些研究假设没有原生生物。
Nucleopore filters of 0.6-1.0 .mu.m pore size have been used to prepare "protist-free" water for a number of studies in microbial ecology. This procedure has been called into question by a recent study claiming that a significant portion of bacterial loss in filtrates could be due to uncharacterized predators passing through 0.6 .mu.m filters. We were unable to directly observe protists in 0.6 .mu.m filtrates using phase contrast, epifluorescence, or transmission electron microscopy. Using the culture techniques of rice grain enrichment and most probable number, however, we were able to observe and quantify several species of bacterivorous nanoflagellates that developed not only in 0.6 .mu.m, but also in 0.4 .mu.m seawater filtrates. The ability of predacious nanoflagellates to squeeze through bacteria-sized pores questions studies of bacterial production and chemical cycling that have assumed protist-free filtrates.