Mass spectrometric evidence for the deficiency in the dark-color-inducing hormone,.
Mass spectrometric evidence for the deficiency in the dark-color-inducing hormone,.
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深色诱导激素缺乏的质谱证据。
DOI：
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发表时间：
2001
影响因子：
2.2
通讯作者：
L. Schoofs
中科院分区：
文献类型：
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作者：
G. Baggerman;E. Clynen;R. Mazibur;D. Veelaert;M. Breuer;A. De Loof;S. Tanaka;L. Schoofs
最近从正常着色蝗虫的心体中分离并鉴定了一种存在于脑和心体中的因子，该因子负责诱导蝗虫的深色。使用来自冲绳（日本）菌株的实验室菌落的白化病突变体监测该因子（命名为[His 7]-corazonin）的纯化。在这项研究中，我们提供了明确的质谱证据表明，大脑和这个白化病蝗虫突变体的心体是缺乏[His 7]-corazonin。以前，[His 7]-corazonin被证明是负责诱导黑暗的体色模式，如在拥挤的蝗虫中观察到的。使用纳米流液相色谱-质谱，我们证明了这种深色诱导激素，但是，存在于语料库cardiaca的孤独蝗虫（Schistocerca gregaria）。Arch.
A factor present in the brain and corpus cardiacum responsible for the induction of dark colour in Locusta migratoria was recently isolated and identified from the corpora cardiaca of normally pigmented locusts. The purification of this factor, designated as [His7]-corazonin was monitored using an albino mutant from a laboratory colony of an Okinawa (Japan) strain. In this study, we provide unequivocal mass spectrometric evidence that the brain and the corpora cardiaca of this albino Locusta mutant are deficient in [His7]-corazonin. Previously, [His7]-corazonin was shown to be responsible for the induction of dark body colour patterns as observed in crowded locusts. Using nanoflow-liquid chromatography-mass spectrometry, we demonstrated that this dark colour-inducing hormone is, however, present in the corpora cardiaca of solitary locusts (Schistocerca gregaria). Arch.