Survival of Schwann cells in chronically denervated skeletal muscles
Survival of Schwann cells in chronically denervated skeletal muscles
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DOI：
10.1007/s00401-001-0504-6
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发表时间：
2002-06-01
影响因子：
12.7
通讯作者：
Carlson, BM
中科院分区：
文献类型：
--
作者：
Dedkov, EI;Kostrominova, TY;Carlson, BM
已经确定，随着时间的推移，雪旺细胞从慢性横断坐骨神经干远端残端的神经内膜间隙中消失。然而，在横断坐骨神经的终末分支内的施瓦尔细胞的状态仍然知之甚少。为了阐明这一问题，我们研究了大鼠后肢骨骼肌肌内神经的神经内膜空间，这已经被去神经支配25个月。基于特定的超微结构特征，我们确定了一个小种群的肌内神经干内的可行施沃尔姆细胞。存活的雪旺细胞继续为S-100蛋白和神经细胞粘附分子的免疫阳性。此外，逆转录-聚合酶链反应和/或蛋白质印迹分析表明，至少有两种分子，脑源性神经营养因子和酪氨酸蛋白激酶受体B的非催化截短形式，这可能潜在地参与神经修复的过程中，可检测到在慢性失神经骨骼肌。我们的结果表明，施沃尔细胞可以在无轴突接触的情况下在去神经骨骼肌的肌内神经干内存活25个月。
It is well established that over time Schwann cells disappear from the endoneurial space of the distal stump of a chronically transected sciatic nerve trunk. Nevertheless, the status of the Schwarm cells within terminal branches of the transected sciatic nerve remains poorly understood. To elucidate this issue we examined the endoneurial space of the intramuscular nerves in rat hindlimb skeletal muscles, which had been denervated for a 25-month period. Based on specific ultrastructural characteristics, we identified a small population of viable Schwarm cells within the intramuscular nerve trunks. The surviving Schwann cells continued to be immunopositive for both S-100 protein and neural cell adhesion molecule. In addition, reverse transcription-polymerase chain reaction and/or Western blot analyses have shown that at least two molecules, brain-derived neurotrophic factor and a non-catalytic truncated form of tyrosine protein kinase receptor B, which could potentially participate in the process of nerve repair, were detectable in chronically denervated skeletal muscle. Our results demonstrate that Schwarm cells can survive inside the intramuscular nerve trunks of denervated skeletal muscle for a 25-month period without axonal contact.