In vitro generation of suppressor cell activity: suppression of in vitro induction if cell-mediated cytotoxicity.
In vitro generation of suppressor cell activity: suppression of in vitro induction if cell-mediated cytotoxicity.
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抑制细胞活性的体外产生：如果细胞介导的细胞毒性，则抑制体外诱导。
DOI：
10.4049/jimmunol.116.1.167
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发表时间：
1976
影响因子：
4.4
通讯作者：
K. Hathcock
中科院分区：
文献类型：
--
作者：
R. Hodes;K. Hathcock
我们观察到，正常小鼠脾细胞在体外单独培养（预培养）3 ~ 7天后，这些细胞在随后的异体脾细胞、三硝苯基（TNP）修饰的同基因脾细胞或同基因肿瘤细胞的体外致敏过程中失去了产生细胞介导的细胞毒性（CML）的能力。这些预先培养的细胞本身不能产生CML，但在混合实验中也显示，它们积极抑制新鲜外植的脾细胞产生CML。抑制发生在CML的致敏期，而不是效应期；抑制性预培养细胞的上清液无抑制性。在预培养前后，用T细胞特异性兔抗小鼠血清和补体（RalphaMB+C）处理脾脏细胞完全消除了抑制作用，这表明T细胞对抑制活性的产生及其表达都是必不可少的。在预培养的尼龙羊毛柱非贴壁脾细胞中，抑制活性完全不存在，这是一个富含T细胞的群体，在脾脏中含有大多数RalphaMB+ c敏感细胞。预培养的尼龙柱贴壁细胞（T细胞缺失）确实具有抑制活性，尼龙贴壁细胞和尼龙非贴壁细胞的混合物在预培养后与预培养的未分离脾脏一样具有抑制活性。此外，在预培养过程中，尼龙粘附脾细胞产生抑制活性的能力被RalphaMB+C处理所破坏。因此，cml抑制活性的“自发”产生依赖于在尼龙柱粘附部分中分离的有限的脾T细胞亚群。讨论了这些数据与先前描述的正常脾脏亚群之间在CML产生中的协同作用的关系，以及与文献中描述的其他抑制系统相关的发现。
It was observed that when normal mouse spleen cells were cultured alone in vitro (precultured) for 3 to 7 days, these cells lost the ability to generate cell-mediated cytotoxicity (CML) during subsequent in vitro sensitization with allogeneic spleen cells, trinitrophenyl (TNP)-modified syngeneic spleen cells, or syngeneic tumor cells. These precultured cells, which were themselves unable to generate CML, were also shown in mixing experiments to suppress, actively, the generation of CML by freshly explanted spleen cells. Suppression occurred at the sensitization phase of CML, and not at the effector level; supernatants from suppressive precultured cells were not suppressive. Suppression was totally abrogated by the treatment of spleen cells with a T cell-specific rabbit anti-mouse brain serum and complement (RalphaMB+C) either before or after preculturing, suggesting that a T cell eas essential both to the generation of suppressor activity and to its expression. Suppressor activity was entirely absent in precultured nylon wool column-nonadherent spleen cells, a T cell-enriched population containing most of the RalphaMB+C-sensitive cells in the spleen. Precultured nylon column-adherent cells (T cell-depleted) did have suppressive activity, and a mixture of nylon-adherent and nylon-non-adherent cells was a suppressive after preculture as the precultured unseparated spleen. Moreover, the ability of nylon-adherent spleen cells to generate suppressive activity during preculturing was abrogated by treatment with RalphaMB+C. Thus, the "spontaneous" generation of CML-suppressive activity was dependent upon a limited subpopulation of splenic T cells isolated in the nylon column-adherent fraction. The relationship of these data to a previously described synergy between subpopulations of normal spleen in the generation of CML is discussed, and the findings related to other suppressor systems described in the literature.