Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter
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DOI：
10.1038/s41598-020-65931-z
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发表时间：
2020-06-01
影响因子：
4.6
通讯作者：
Nakagata, Naomi
中科院分区：
文献类型：
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作者：
Nakao, Satohiro;Takeo, Toru;Nakagata, Naomi
通过流式细胞术进行细胞分选是许多生物领域中筛选细胞亚群的有力工具。选择容易受精的精子是确保体外受精(IVF)中稳定和高受精率的关键步骤。然而，由于在分选过程中精子受到严重的机械损伤，常规细胞分选和体外受精系统的结合尚未建立起来。一种具有微流控芯片技术的细胞分选机可以解决这个问题，该技术可以减少细胞分选期间的细胞损伤。通过对微流控芯片细胞分选对精子活力、受精率等参数的评价，发现这种细胞分选方法对精子的危害最小。然后，通过顶体反应的标记选择精子，并显示出比顶体完整精子群体更高的受精率。从这些精子中获得的胚胎发育正常。这些结果表明，微流控芯片细胞分选技术可以筛选出有生育能力的精子，从而提高体外受精技术。
Cell sorting via flow cytometry is a powerful tool to select subpopulations of cells in many biological fields. Selection of fertilisation-prone sperm is a critical step to ensure a stable and high fertilisation rate in in vitro fertilisation (IVF). However, a combination of conventional cell sorting and IVF system has not been established because of severe mechanical damages to the sperm during the sorting process. A cell sorter with microfluidics chip technology that lessens cell damage during cell sorting may address this problem. We evaluated the effects of microfluidics chip cell sorting on the sperm using the parameters, such as motility and fertility, and found this cell sorting method had minimal harmful effect on the sperm. Then, sperm were selected by a marker for acrosome reaction and showed higher fertilisation rate than that of the population of acrosome-intact sperm. Embryo derived from these sperm developed normally. These results indicated that microfluidics chip cell sorting can select fertile sperm to improve IVF technique.