Immunomagnetic separation of adult human olfactory neural progenitors.
Immunomagnetic separation of adult human olfactory neural progenitors.
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成人嗅觉神经祖细胞的免疫磁性分离。
DOI：
10.1080/10520290500469769
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Roisen,Fj
中科院分区：
文献类型：
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作者：
Othman,M;Klueber,K;Lu,C;Winstead,W;Roisen,Fj
嗅神经上皮细胞（ONe）由于存在有丝分裂活跃的祖细胞而具有终身再生能力。ONe的可及性使其成为CNS中细胞替代策略的祖细胞的独特来源。我们已经建立了神经球形成细胞（NSFCs）线从成人死后ONe和患者接受鼻窦手术的内窥镜活检。这些异质线主要由一个不成熟的神经元限制和一个小的神经胶质限制的亚群。更同质的NSFCs亚群是必不可少的详细研究影响其谱系限制的因素。使用抗酪氨酸激酶（Trk）受体（Trk-pan，其识别Trk-A、B、C）的抗体的免疫磁珠分离导致可以进行免疫细胞化学分析的活的、富集的阳性和阴性亚群。阳性细胞在第一周保持阳性，之后Trk-pan表达细胞的数量减少。阴性亚群在体外培养5天后开始表达Trk-pan免疫反应性。两周后，两个亚群都恢复了异质组成。此外，大多数NSFC对Trk-B呈阳性，少数对Trk-A呈阳性，而对Trk-C无反应性。由于NSFC产生脑源性神经营养因子（BDNF）并表达BDNF的特异性受体Trk B，因此群体动态可能处于旁分泌和/或自分泌调节机制下。谱系限制分析表明，孤立的亚群具有限制潜力相当于原来的异质性人口。这些研究进一步表征了NSFC，并支持ONe衍生的祖细胞未来用于CNS损伤和神经退行性疾病的潜在治疗用途。
Olfactory neuroepithelium (ONe) has lifelong regenerative capacity owing to the presence of mitotically active progenitors. The accessibility of ONe makes it a unique source of progenitors for cell replacement strategies in the CNS. We have established lines of neurosphere forming cells (NSFCs) from adult postmortem ONe and patients undergoing nasal sinus surgery by endoscopic biopsy. These heterogeneous lines are composed primarily of an immature neuronally restricted and a small glial restricted subpopulation. More homogeneous subpopulations of the NSFCs are essential for detailed study of factors influencing their lineage restriction. Immunomagnetic bead separation using an antibody against tyrosine kinase (Trk) receptors (Trk-pan, which recognizes Trk-A, B, C) resulted in viable, enriched positive and negative subpopulations that could be analyzed immunocytochemically. The positive cells remained positive for the first week after which the number of Trk-pan expressing cells decreased. The negative subpopulation began to express Trk-pan immunoreactivity after five days in vitro. Both subpopulations reverted to the heterogeneous composition after two weeks. Furthermore, most NSFCs were positive for Trk-B, a few for Trk-A, while no reactivity was observed for Trk-C. Because NSFCs produce brain derived neurotrophic factor (BDNF) and express Trk B, the specific receptor for BDNF, it is likely that population dynamics are under a paracrine and/or autocrine regulatory mechanism. Lineage restriction analysis demonstrated that the isolated subpopulation had a restriction potential equivalent to the original heterogeneous population. These studies characterize further the NSFCs and support the future potential therapeutic use of ONe-derived progenitors for CNS injury and neurodegenerative disorders.