Cardiovascular Risk Factors Associated With Blood Metabolite Concentrations and Their Alterations During a 4-Year Period in a Population-Based Cohort
Cardiovascular Risk Factors Associated With Blood Metabolite Concentrations and Their Alterations During a 4-Year Period in a Population-Based Cohort
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DOI：
10.1161/circgenetics.116.001444
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发表时间：
2016-12-01
影响因子：
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通讯作者：
Haerting, Johannes
中科院分区：
文献类型：
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作者：
Lacruz, Maria Elena;Kluttig, Alexander;Haerting, Johannes
背景-迄今为止，生活方式风险因素对人体代谢的影响尚不清楚。我们的目的是调查这些危险因素与代谢物及其变化的关联，在4 years.Methods和Results-One和63代谢物测定血清样本中的AbsoluteIDQ试剂盒p150（Biocrates）以下有针对性的代谢组学方法，在一个人口为基础的队列1030个人，年龄在基线45至83岁。我们评估了代谢物浓度（28种酰基肉毒碱，14种氨基酸，9种溶血磷酸胆碱，72种磷酸胆碱，10种鞘磷脂和己糖总和）与5种生活方式风险因素（体重指数[BMI]，饮酒，吸烟，饮食和运动）之间的相关性。调整相关协变量的多水平或简单线性回归模型分别用于评估横截面或纵向关联;多重检验校正基于错误发现率。BMI、饮酒和吸烟与脂质代谢相关（溶血和酰基烷基磷脂酰胆碱减少，二酰基磷脂酰胆碱浓度增加）。吸烟与酰基肉毒碱呈正相关，BMI与非必需氨基酸呈负相关。较少的代谢物显示出与基线风险因素相关的相对变化：5种不同酰基烷基磷脂酰胆碱的增加与较低的饮酒量和BMI以及更健康的饮食相关。酪氨酸水平升高与BMI相关。吸烟和体重指数的性别特异性影响被发现具体相关acylcarnitine代谢：在女性更高的体重指数和男性更多包年与acylcarnitines. Conclusions增加，这项研究表明，性别特异性影响的生活方式风险因素对人体代谢，并强调其长期的代谢后果。
Background-The effects of lifestyle risk factors considered collectively on the human metabolism are to date unknown. We aim to investigate the association of these risk factors with metabolites and their changes during 4 years.Methods and Results-One hundred and sixty-three metabolites were measured in serum samples with the AbsoluteIDQ kit p150 (Biocrates) following a targeted metabolomics approach, in a population-based cohort of 1030 individuals, aged 45 to 83 years at baseline. We evaluated associations between metabolite concentrations (28 acylcarnitines, 14 amino acids, 9 lysophosphocholines, 72 phosphocholines, 10 sphingomyelins and sum of hexoses) and 5 lifestyle risk factors (body mass index [BMI], alcohol consumption, smoking, diet, and exercise). Multilevel or simple linear regression modeling adjusted for relevant covariates was used for the evaluation of cross-sectional or longitudinal associations, respectively; multiple testing correction was based on false discovery rate. BMI, alcohol consumption, and smoking were associated with lipid metabolism (reduced lyso-and acyl-alkyl-phosphatidylcholines and increased diacylphosphatidylcholines concentrations). Smoking showed positive associations with acylcarnitines, and BMI correlated inversely with nonessential amino acids. Fewer metabolites showed relative changes that were associated with baseline risk factors: increases in 5 different acyl-alkyl phosphatidylcholines were associated with lower alcohol consumption and BMI and with a healthier diet. Increased levels of tyrosine were associated with BMI. Sex-specific effects of smoking and BMI were found specifically related to acylcarnitine metabolism: in women higher BMI and in men more pack-years were associated with increases in acylcarnitines.Conclusions-This study showed sex-specific effects of lifestyle risks factors on human metabolism and highlighted their long-term metabolic consequences.