Antimicrobial peptides on calcium phosphate-coated titanium for the prevention of implant-associated infections
Antimicrobial peptides on calcium phosphate-coated titanium for the prevention of implant-associated infections
复制标题
DOI：
10.1016/j.biomaterials.2010.08.035
复制
发表时间：
2010-12-01
期刊：
影响因子：
14
通讯作者：
Wang, Rizhi
中科院分区：
文献类型：
--
作者：
Kazemzadeh-Narbat, Mehdi;Kindrachuk, Jason;Wang, Rizhi
预防植入物相关感染一直是骨科手术的主要挑战之一，由于使用传统抗生素预防感染而导致抗生素耐药性的发展，这一挑战进一步复杂化。本研究的目的是开发一种能够负载和局部递送一组独特的阳离子抗菌肽（AMP）的技术。通过电解沉积法在钛表面制备一薄层微孔磷酸钙（CaP）涂层作为药物载体。（KRWWKWWRRC）并负载到CaP涂层上，SEM XRD和FTIR分析证实CaP涂层是微纳米的。多孔磷酸八钙通过使用发光光谱仪技术，证明了使用简单的浸泡技术，7 μ m厚的多孔CaP涂层可以负载高达9 μ g AMP/cm（2）。（CaP-Tet 213）对MG-63成骨细胞样细胞没有细胞毒性。（金黄色葡萄球菌）和革兰氏阴性（铜绿假单胞菌）细菌，如通过测量菌落形成单位（CFU）评估的，两种细菌菌株在30分钟内减少10（6）倍。Tet 213样品通过四个测试循环证明了CaP-Tet 213表面的抗微生物活性的保留。还通过评估在4小时和24小时对含有luxCDABE盒的铜绿假单胞菌的发光的抑制来评价细菌对CaP-Tet 213表面的敏感性，其相似性为92%和相似性为77%。在CaP植入物与等摩尔浓度的Tet 213、商业开发的抗微生物肽MX-226 hLF 1 -11或CaP-妥布霉素孵育后，证明了CaP-Tet 213是比CaP-MX-226、CaP-hLF 1 -11或CaP-妥布霉素更有效的抗微生物涂层。11或妥布霉素A的装置与CaP-Tet 213涂层可能是一种潜在的解决方案，用于预防种植体周围感染的骨科（C）2010 Elsevier Ltd版权所有
Prevention of implant-associated infections has been one of the main challenges in orthopaedic surgery This challenge is further complicated by the concern over the development of antibiotic resistance as a result of using traditional antibiotics for infection prophylaxis The objective of this study was to develop a technique that enables the loading and local delivery of a unique group of cationic antimicrobial peptides (AMP) through implant surfaces A thin layer of micro-porous calcium phosphate (CaP) coating was processed by electrolytic deposition onto the surface of titanium as the drug carrier The broad spectrum AMP Tet213 (KRWWKWWRRC) was selected and loaded onto the CaP coating SEM XRD and FTIR analyses confirmed the CaP coating to be micro-porous octacalcium phosphate By using a luminescence spectrometer technique it was demonstrated that a 7 mu m thick porous CaP coating could load up to 9 mu g of AMP/cm(2) using a simple soaking technique The drug-loaded CaP coating (CaP-Tet213) was not cytotoxic for MG-63 osteoblast-like cells The CaP-Tet213 implants had antimicrobial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with 10(6)-fold reductions of both bacterial strains within 30 min as assessed by measuring colony-forming units (CFU) Repeated CFU assays on the same CaP-Tet213 specimen demonstrated retention of antimicrobial activity by the CaP-Tet213 surfaces through four test cycles The susceptibility of bacteria to the CaP-Tet213 surfaces was also evaluated by assessing the inhibition of luminescence of P aeruginosa containing a luxCDABE cassette at 4 h and 24 h with similar to 92% and similar to 77% inhibition of luminescence respectively It was demonstrated that CaP-Tet213 was a more efficient antimicrobial coating than CaP-MX226 CaP-hLF1-11 or CaP-tobramycin following incubation of CaP implants with equimolar concentrations of Tet213 the commercially developed antimicrobial peptide MX-226 hLF1-11 or tobramycin A device coated with CaP-Tet213 could be a potential solution for the prevention of the peri-implant infection in orthopaedics (C) 2010 Elsevier Ltd All rights reserved