White button mushroom phytochemicals inhibit aromatase activity and breast cancer cell proliferation
White button mushroom phytochemicals inhibit aromatase activity and breast cancer cell proliferation
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DOI：
10.1093/jn/131.12.3288
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发表时间：
2001-12-01
影响因子：
4.2
通讯作者：
Chen, S
中科院分区：
文献类型：
--
作者：
Grube, SJ;Eng, ET;Chen, S
雌激素是乳腺癌发生的主要因素。乳腺癌中芳香族酶/雌激素合成酶的原位雌激素合成在肿瘤增殖中起主导作用。由于黄酮类和异黄酮类等天然化合物已被证明是芳香酶的抑制剂，因此人们认为，含有这些植物化学物质的蔬菜可以抑制芳香酶活性，抑制乳腺癌细胞的增殖。从蔬菜中制备了热稳定的提取物，并在人胎盘微粒体试验中筛选了它们抑制芳香酶活性的能力。双孢菇对芳香酶活性的抑制作用呈剂量依赖关系。酶动力学表现为混合抑制，提示存在多种抑制物或多种抑制机制。用芳香酶基因转导的乳腺癌细胞株MCF-7aro检测细胞内芳香化酶活性和细胞增殖。香菇水提物中的植物化学物质可抑制MCF-7aro细胞的芳香化酶活性和增殖。这些结果表明，高蘑菇饮食可能通过减少雌激素的原位产生来调节绝经后妇女化学预防中芳香化酶的活性和功能。
Estrogen is a major factor in the development of breast cancer. In situ estrogen production by aromatase/estrogen synthetase in breast cancer plays a dominant role in tumor proliferation. Because natural compounds such as flavones and isoflavones have been shown to be inhibitors of aromatase, it is thought that vegetables that contain these phytochemicals can inhibit aromatase activity and suppress breast cancer cell proliferation. Heat-stable extracts were prepared from vegetables and screened for their ability to inhibit aromatase activity in a human placental microsome assay. The white button mushroom (species Agaricus bisporus) suppressed aromatase activity dose dependently. Enzyme kinetics demonstrated mixed inhibition, suggesting the presence of multiple inhibitors or more than one inhibitory mechanism. "In cell" aromatase activity and cell proliferation were measured using MCF-7aro, an aromatase-transfected breast cancer cell line. Phytochemicals in the mushroom aqueous extract inhibited aromatase activity and proliferation of MCF-7aro cells. These results suggest that diets high in mushrooms may modulate the aromatase activity and function in chemoprevention in postmenopausal women by reducing the in situ production of estrogen.