EVIDENCE OF RESTRICTED VIRAL REPLICATION IN ADULT MINK INFECTED WITH ALEUTIAN DISEASE OF MINK PARVOVIRUS
EVIDENCE OF RESTRICTED VIRAL REPLICATION IN ADULT MINK INFECTED WITH ALEUTIAN DISEASE OF MINK PARVOVIRUS
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DOI：
10.1128/jvi.62.5.1495-1507.1988
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发表时间：
1988-05-01
影响因子：
5.4
通讯作者：
WOLFINBARGER, J
中科院分区：
文献类型：
--
作者：
ALEXANDERSEN, S;BLOOM, ME;WOLFINBARGER, J
用链特异性杂交探针进行原位分子杂交，对水貂细小病毒阿留申病（ADV）复制中间体细胞进行了特异性定位。当阿留申基因型的成年水貂感染ADV犹他州I时，病毒复制阳性的细胞数量最多（即，在感染后10天，在肠系膜淋巴结和脾脏中发现了含有复制型DNA和RNA的病毒。阳性细胞定位于生发中心中部，提示为B淋巴母细胞。循环中的白细胞和骨髓细胞也含有病毒RNA，但复制型DNA的水平低于可检测性。与允许感染的细胞培养物或新生水貂相比，复制ADV的成年水貂细胞中的病毒DNA和RNA水平下降，这表明ADV在成年水貂中的复制可能在病毒基因表达的早期阶段是半允许或限制的。淋巴细胞中病毒复制和转录水平低可能为免疫紊乱的发展和持续感染的维持提供了机制。在其他器官中也发现了单链病毒体DNA，但链特异性探针使得有可能表明这种DNA代表病毒隔离。此外，肾小球免疫复合物含有病毒粒子DNA检测，表明ADV病毒粒子，或可能游离DNA，可能有一个作用，在发展中的ADV诱导的肾小球肾炎。
Strand-specific hybridization probes were used in in situ moelcular hybridization specifically to localize cells containing replicative intermediates of Aleutian disease of mink parvovirus (ADV). When adult mink of Aleutian genotype were infected with ADV Utah I, the largest number of cells positive for viral replication (i.e., containing replicative-form DNA and RNA) were found in the mesenteric lymph nodes and spleens at 10 days after infection. The localization of positive cells in the middle of germinal centers suggested that they were B lymphoblasts. Circulating leukocytes and bone marrow cells also contained viral RNA, but the levels of replicative-form DNA were below detectability. The levels of viral DNA and RNA in adult mink cells replicating ADV were decreased compared with those in permissively infected cell cultures or neonatal mink, suggesting that the replication of ADV in adult mink might be semipermissive or restricted at some early stage of viral gene expression. The low level of viral replication and transcription in lymphoid cells might provide a mechanism for the development of immune disorders and for the maintenance of persistent infection. single-stranded virion DNA was found in other organs, but the strand-specific probes made it possible to show that this DNA represented virus sequestration. In addition, glomerular immune complexes containing virion DNA were detected, suggesting that ADV virions, or perhaps free DNA, may have a role in the development of ADV-induced glomerulonephritis.