Altered expression pattern of alternatively spliced estrogen receptor β transcripts in breast carcinoma
Altered expression pattern of alternatively spliced estrogen receptor β transcripts in breast carcinoma
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DOI：
10.1016/j.canlet.2004.05.006
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发表时间：
2004-11-08
期刊：
影响因子：
9.7
通讯作者：
Lidereau, R
中科院分区：
文献类型：
--
作者：
Girault, I;Andrieu, C;Lidereau, R
总雌激素受体β（ER β）表达失调与乳腺肿瘤发生有关。ERP基因产生5个外显子8选择性剪接转录本（ER β 1 -5），其编码具有不同C-末端氨基酸的蛋白质。这些转录本的个体表达分析可能为乳腺癌中雌激素信号转导提供新的见解。我们通过实时逆转录-聚合酶链反应和荧光片段分析测定了正常组织、绝经后乳腺癌患者和乳腺癌细胞系中总ER β及其5种亚型的mRNA水平。在各种正常人体组织中，ER β 1 -5亚型显示出不同的定性和定量表达模式，这与以前的报道一致。乳腺肿瘤组织中总ERP mRNA水平显著低于正常乳腺组织（38倍，P
Dysregulation of total estrogen receptor beta (ERbeta) expression has been implicated in breast tumorigenesis. The ERP gene yields five exon 8 alternatively spliced transcripts (ERbeta1-5), which encode proteins with different C-terminal amino acids. Individual expression analysis of these transcripts may provide new insights into estrogen signaling in breast cancer. We measured mRNA levels of total ERbeta and its five isoforms in normal tissues, breast carcinomas from post-menopausal patients, and breast cancer cell lines by means of real-time reverse transcription-polymerase chain reaction and fluorescent fragment analysis. In various normal human tissues, ERbeta1-5 isoforms displayed different qualitative and quantitative expression patterns that were consistent with previous reports. Total ERP mRNA levels were significantly lower in breast tumors than in normal breast tissues (38-fold lower, P