Induction in mouse peritoneal macrophages of 34 kDa stress protein and heme oxygenase by sulfhydryl-reactive agents.
Induction in mouse peritoneal macrophages of 34 kDa stress protein and heme oxygenase by sulfhydryl-reactive agents.
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巯基反应剂诱导小鼠腹腔巨噬细胞产生 34 kDa 应激蛋白和血红素加氧酶。
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
S. Bannai
中科院分区：
文献类型：
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作者：
S. Taketani;H. Sato;T. Yoshinaga;R. Tokunaga;T. Ishii;S. Bannai
当小鼠巨噬细胞暴露于顺丁烯二酸二乙酯或亚砷酸钠时，34 kDa应激蛋白的合成增加，同时血红素加氧酶活性增加。在巯基试剂作用7h后，34 kDa的蛋白质是合成最活跃的蛋白质。免疫印迹分析表明，诱导表达的34 kDa蛋白可与抗牛血红素加氧酶抗体反应。镉离子或1-氯-2，4-二硝基苯也能诱导与抗体发生反应的34 kDa蛋白。丁硫氨酸亚硫胺或过氧化氢处理细胞后，对该蛋白的诱导作用较弱，而联胺或热休克处理则不起作用。这些结果与我们以前的发现是一致的，即重金属离子包括亚砷酸盐和镉离子诱导人类细胞系[Taketani，S.，Kohno，H.，Yoshinaga，T.和Tokunaga，R.(1989)FEBS Lett]中的血红素加氧酶(32 kDa应激蛋白)。245,173-176]，并提示谷胱甘肽与羟基反应物的结合可能介导了小鼠腹腔巨噬细胞应激蛋白的诱导。
The synthesis of 34-kDa stress protein was enhanced, with a simultaneous increase in heme oxygenase activity, when mouse macrophages were exposed to diethylmaleate or sodium arsenite. After 7 h of exposure to the sulfhydryl agents, the 34-kDa protein was the most actively synthesized protein. Immunoblot analysis showed that the induced 34-kDa protein reacted with an antibody raised against bovine heme oxygenase. Cadmium ions or 1-chloro-2,4-dinitrobenzene also induced the 34-kDa protein which reacted with the antibody. Treatments of the cells with buthionine sulfoximine or hydrogen peroxide weakly induced the protein, while diamide treatment or heat shock was without effect. These results are consistent with our previous findings that heavy metal ions including arsenite and cadmium ions induce heme oxygenase (32-kDa stress protein) in human cell lines [Taketani, S., Kohno, H., Yoshinaga, T., & Tokunaga, R. (1989) FEBS Lett. 245, 173-176], and also suggest that the formation of glutathione conjugate with sulfhydryl-reactive agents may mediate the induction of the stress protein in mouse peritoneal macrophages.