Antigen presentation by murine epidermal langerhans cells and its alteration by ultraviolet B light.
Antigen presentation by murine epidermal langerhans cells and its alteration by ultraviolet B light.
复制标题
鼠表皮朗格汉斯细胞的抗原呈递及其通过紫外线 B 光的改变。
作者：
G. Stingl;L. A. Gazze;W. Aberer;K. Wolff
在体内长期暴露于紫外线B光(UV-B)的小鼠对各种抗原刺激表现出不同的免疫反应性。UV-B作用的一种可能模式是它对抗原提呈细胞功能产生不利影响。由于表皮是自然受到紫外线照射的唯一组织，我们调查了小鼠表皮细胞(EC)是否可以执行抗原递送功能，如果是，这种功能是否可以通过UV-B辐射来改变。为此，来自BALB/c或C3H/He小鼠的纯化蛋白衍生物(PPD)和二硝基苯基卵清蛋白(DNP/sub6/-OVA)免疫的T细胞与同基因、半同基因或异基因EC孵育，或者作为对照，与脉冲暴露于免疫抗原或未冲击或冲击人血清白蛋白(HSA)的腹膜渗出细胞(PEC)孵育。培养4d后，用/sup-3/H-胸腺嘧啶核苷掺入法检测T细胞增殖情况。PPD和DNP/6-OVA冲击，而不是HSA冲击的EC和PEC，诱导同基因和半同基因免疫T细胞的强烈增殖，但不能诱导同种异体免疫T细胞的增殖。用特异性抗Ia血清和补体预刺激刺激细胞几乎完全消除了这种反应，这表明在EC中，携带Ia的朗格汉斯细胞是关键的刺激细胞。在UV-B照射前后，EC对抗原特异性T细胞增殖均有剂量依赖性的损伤作用，20mJ/cm/sup 2/UV-B照射后，T细胞增殖反应消失。在所使用的剂量范围内的UV-B不会立即造成致命的细胞损伤、培养的EC过早死亡或抑制T细胞增殖的毒性因素。
Mice that are chronically exposed in vivo to ultraviolet B light (UV-B) display altered immunologic reactivity to various antigenic stimuli. A possible mode of UV-B action is that it exerts adverse effects on antigen-presenting cell function. Because the epidermis is the only tissue that is naturally subject to UV exposure we investigated if murine epidermal cells (EC) could perform an antigen presentation function and, if so, could this function be altered by UV-B irradiation. For this purpose, T cells immune to purified protein derivative of tuberculin (PPD) and dinitrophenylated ovalbumin (DNP/sub 6/-OVA) from either BALB/c or C3H/He mice were incubated with syngeneic, semisyngeneic, or allogeneic EC or, for control purposes, with peritoneal exudate cells (PEC) that had been pulse-exposed to either the immunizing antigens or, as controls, left unpulsed, or pulsed to human serum albumin (HSA). After 4 days of culture, T cell proliferation was assessed by /sup 3/H-thymidine incorporation. PPD- and DNP/6-OVA pulsed, but not HSA-pulsed EC and PEC, induced vigorous proliferation of syngeneic and semisyngeneic, but not allogeneic, immune T cells. Pretreatment of stimulator cells with specific anti-Ia serum and complement virtually abolished this response, which indicated that among EC, Ia-bearing Langerhans cells are the critical stimulators. Exposuremore » of EC either before or after pulsing to UV-B resulted in a dose-dependent impairment of antigen-specific T cell proliferation; the T proliferative response was abolished after administration of 20 mJ/cm/sup 2/ UV-B. UV-B in the dose range employed did not produce immediate lethal cell damage, premature death of cultured EC, or toxic factors inhibitory for T cell proliferation.« less