Nucleolar clustering of dispersed tRNA genes
Nucleolar clustering of dispersed tRNA genes
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DOI：
10.1126/science.1089814
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发表时间：
2003-11-21
期刊：
影响因子：
56.9
通讯作者：
Engelke, DR
中科院分区：
文献类型：
--
作者：
Thompson, M;Haeusler, RA;Engelke, DR
酿酒酵母中的早期转移RNA(TRNA)加工事件是在核仁中协调的，核仁通常与核糖体生物合成有关。为了检验tRNA途径的空间组织是否始于基因的核仁聚集，我们探索了五个不同tRNA基因家族的亚核位置。结果表明，tRNA基因虽然分散在线性基因组中，但在核仁上与5S核糖体DNA和U14小核仁RNA共定位。核仁定位需要tRNA基因转录复合体的形成，因为单个位点的启动子失活会消除其核仁关联。TRNA基因的这种组织肯定深刻地影响了基因组的空间包装，并提出了一个问题，即基因类型是否可能在三维空间中协调以调节转录。
Early transfer RNA (tRNA) processing events in Saccharomyces cerevisiae are co-ordinated in the nucleolus, the site normally associated with ribosome biosynthesis. To test whether spatial organization of the tRNA pathway begins with nucleolar clustering of the genes, we have probed the subnuclear location of five different tRNA gene families. The results show that tRNA genes, though dispersed in the linear genome, colocalize with 5S ribosomal DNA and U14 small nucleolar RNA at the nucleolus. Nucleolar localization requires tRNA gene transcription-complex formation, because inactivation of the promoter at a single locus removes its nucleolar association. This organization of tRNA genes must profoundly affect the spatial packaging of the genome and raises the question of whether gene types might be coordinated in three dimensions to regulate transcription.