Regulation of the depth of surface liquid in bovine trachea
Regulation of the depth of surface liquid in bovine trachea
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DOI：
10.1152/ajplung.1998.274.3.l388
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发表时间：
1998-03-01
影响因子：
4.9
通讯作者：
Widdicombe, JH
中科院分区：
文献类型：
--
作者：
Wu, DXY;Lee, CYC;Widdicombe, JH
气道的腔表面衬有气道表面液体（ASL）的薄膜。ASL深度的生理调节以前没有报道。在本文中，我们已经使用低温扫描电子显微镜的快速冷冻标本的牛气管上皮细胞，以证明改变的深度ASL的胆碱能激动剂乙酰甲胆碱。我们首先建立了乙酰甲胆碱选择性刺激气道腺体，最大分泌在类似2分钟，并返回到基线内类似5分钟。2分钟暴露于乙酰甲胆碱增加了ASL的深度从23到78 μ m。此后，深度随时间线性下降，达到32 μ m，在30分钟。在深度的初始增加被布美他尼，抑制剂的活性氯分泌，而缓慢下降回基线抑制阿米洛利，阻断剂的活性钠吸收。我们的结论是乙酰甲胆碱引起的ASL深度的变化反映了短暂的腺体分泌，然后通过表面上皮细胞的液体吸收。
The luminal surface of airways is lined by a thin film of airway surface liquid (ASL). Physiological regulation of the depth of ASL has not been reported previously. In this paper, we have used low-temperature scanning electron microscopy of rapidly frozen specimens of bovine tracheal epithelium to demonstrate alterations in the depth of ASL in response to the cholinergic agonist methacholine. We first established that methacholine selectively stimulated airway glands, with maximal secretion at similar to 2 min and a return to baseline within similar to 5 min. A 2-min exposure to methacholine increased the depth of ASL from 23 to 78 mu m. Thereafter, depth decreased linearly with time, reaching 32 mu m at 30 min. The initial increase in depth was blocked by bumetanide, an inhibitor of active chloride secretion, whereas the slow decline back to baseline was inhibited by amiloride, a blocker of active sodium absorption. We conclude that the methacholine-induced changes in ASL depth reflect transient gland secretion followed by liquid absorption across the surface epithelium.