Immunohistological assessment of intestinal eosinophil activation in patients with eosinophilic gastroenteritis and inflammatory bowel disease
Immunohistological assessment of intestinal eosinophil activation in patients with eosinophilic gastroenteritis and inflammatory bowel disease
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DOI：
10.1111/j.1572-0241.1999.01641.x
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发表时间：
1999-12-01
影响因子：
9.8
通讯作者：
Manns, MP
中科院分区：
文献类型：
--
作者：
Bischoff, SC;Mayer, J;Manns, MP
目的：通过免疫组织化学方法评估嗜酸性粒细胞性胃肠炎（EOG）、溃疡性结肠炎（UC）、克罗恩病（CD）患者和对照患者肠道嗜酸性粒细胞的活化程度。 方法：从健康对照者以及患有EGG、CD、UC和其他非炎症性胃肠道疾病的患者中采集盲肠活检样本。在用单克隆抗体染色的连续切片中进行免疫组织化学，所述单克隆抗体针对储存在嗜酸性粒细胞颗粒（EG1）中和由活化的嗜酸性粒细胞（EG2）分泌的嗜酸性粒细胞阳离子蛋白（ECP）或嗜酸性粒细胞蛋白X（EPX）。计算 EG1 与 EG2 阳性嗜酸性粒细胞的比率，以固有层细胞的百分比表示。检测血清和粪便中的 ECP 和 EPX。结果：EG1 和 EG2 阳性固有层细胞的百分比在 EOG 中升高，在 UC 中略有升高，但不显着。与健康对照相比，CD、UC 和其他患者中 EG1 与 EG2 阳性细胞的比例降低。 EGG 中 EG1 与 EG2 的比例特别低。相应地，EGG 患者的粪便和血清 ECP 和 EPX 水平分别最高。 EG1与EG2的比率与粪便ECP和EPX水平呈负相关。在活动性炎症粘膜部位，EG1 与 EG2 的比率低于非炎症组织。结论：我们的数据强烈表明 EG1 与 EG2 的比率可能是组织嗜酸性粒细胞活化的标志。低比率（
OBJECTIVE: To assess the activation grade of intestinal eosinophils in patients with eosinophilic gastroenteritis (EOG), ulcerative colitis (UC), Crohn's disease (CD), and controls by immunohistochemistry.METHODS: Cecal biopsies were collected from healthy controls and from patients with EGG, CD, UC, and other noninflammatory GI diseases. Immunohistochemistry was performed in sequential sections stained with monoclonal antibodies directed against eosinophil cationic protein (ECP) or eosinophil protein X (EPX) stored in, eosinophil granules (EG1) and that secreted by activated eosinophils (EG2). The ratio of EG1 to EG2-positive eosinophils expressed as percentage of lamina propria cells was calculated. ECP and EPX were measured in serum and feces.RESULTS: The percentage of EG1 and EG2-positive lamina propria cells was elevated in EOG and slightly, but not significantly, in UC. The ratio of EG1 to EG2-positive cells was decreased in CD, UC, and other patients as compared tol healthy controls. Particularly low EG1 to EG2 ratios were found in EGG. Correspondingly, fecal and serum levels of ECP and EPX, respectively, were highest in patients with EGG. The EG1 to EG2 ratio was negatively correlated with fecal ECP and EPX levels. At sites of actively inflamed mucosa, the EG1 to EG2 ratio was lower than in noninflamed tissue.CONCLUSIONS: Our data strongly suggest that the EG1 to EG2 ratio may be a marker of tissue eosinophil activation. Low ratios (