Ectoine production from putrefactive non-volatile amines in the moderate halophile Halomonas elongata
Ectoine production from putrefactive non-volatile amines in the moderate halophile Halomonas elongata
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中度嗜盐菌 Halomonas elongata 中腐败的非挥发性胺产生四氢嘧啶
DOI：
10.1088/1755-1315/439/1/012001
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Miyoshi K
中科院分区：
文献类型：
--
作者：
Nakayama H;Kawamoto R;Miyoshi K
中度嗜盐长臂嗜盐单胞菌可以吸收生物废弃物中的多种糖和/或氨基酸作为碳源和/或氮源。因此，长柄扁豆可能是一种实用的细胞工厂，用于生物生产Ecotoine等精细化学品。在富含蛋白质的生物废弃物中，腐烂的非挥发性胺在生物腐烂过程中通过氨基酸的脱羧基作用形成生物胺，具有相当大的毒理学风险。因此，我们研究了长链藻是否能同化主要腐烂的非挥发胺、组胺和酪胺作为碳源和/或氮源。通过对两个近缘株系OUT30018和DSM2581T的比较分析，发现OUT30018能同化组胺和酪胺，并在高盐度下产生外生碱，而DSM2581T则不能。因此，从富含蛋白质的生物垃圾中提取腐烂的非挥发性胺类化合物，使其成为最有前途的细胞工厂之一。
The moderate halophile Halomonas elongata can assimilate a variety of sugars and/or amino acids derived from bio-wastes as carbon and/or nitrogen sources. Thus, H. elongata could be a practical cell factory for bioproduction of fine chemicals such as ectoine. In protein-rich bio-wastes, putrefactive non-volatile amines could be formed as biogenic amines by decarboxylation of amino acids during biological rotten processes and be a considerable toxicological risk. Therefore, we investigated whether H. elongata can assimilate major putrefactive non-volatile amines, Histamine and Tyramine, as carbon and/or nitrogen sources or not. Comparative analysis using closely related H. elongata strains, OUT30018 and DSM2581 T, revealed that H. elongata OUT30018 can assimilate both Histamine and Tyramine, and produce ectoine under high salinity, while H. elongata DSM2581 T cannot. Thus, H. elongata OUT30018 would be a one of the most promising cell factories to produce fine chemicals such as ectoine from the putrefactive non-volatile amines in protein-rich bio-waste.