Cleavage of The Double-strand Region in Alkali-denatured Supercoiled Plasmid pBR322 Leads to DNA Nodes
Cleavage of The Double-strand Region in Alkali-denatured Supercoiled Plasmid pBR322 Leads to DNA Nodes
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碱变性超螺旋质粒 pBR322 中双链区域的裂解导致 DNA 节点
DOI：
10.16476/j.pibb.2015.0160
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发表时间：
2016-06
影响因子：
0.3
通讯作者：
Huang Xi-Tai
中科院分区：
文献类型：
--
作者：
Tian Lei;Su Xiao-Yun;Zhang Zhen-Feng;Huang Xi-Tai
为进一步研究碱变性超螺旋DNA(Ⅳ型DNA；DNA Ⅳ)的结构，对碱变性质粒pBR322进行了酶学分析并利用原子力显微镜(AFM)对新形成的DNA结构进行了观察和比较.在所用的限制性内切酶中，PstⅠ能切割pBR 3
Combinatorial analyses including enzymatic analysis and atomic force microscopy (AFM) were used to gain more insights into structure of the alkali-denatured plasmid pBR322 DNA (DNA Ⅳ). Among the restriction enzymes tested, PstⅠ was able to cleave the pBR3