Immunochemical study of role of chargerin II, a product of URFA6L of mitochondrial DNA in energy transduction of rat liver mitochondria.
Immunochemical study of role of chargerin II, a product of URFA6L of mitochondrial DNA in energy transduction of rat liver mitochondria.
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线粒体 DNA URFA6L 产物 Chargerin II 在大鼠肝线粒体能量转导中作用的免疫化学研究。
DOI：
10.1016/0006-291x(87)90739-x
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发表时间：
1987
影响因子：
3.1
通讯作者：
T. Higuti
中科院分区：
文献类型：
--
作者：
J. Uchida;M. Takigawa;T. Yamane;T. Negama;I. Tani;T. Higuti
抗chargerin II抗体[线粒体DNA的未知阅读框A6L （URFA6L）的产物]抑制ATP-Pi交换反应，逆转线粒体（内膜加基质）中琥珀酸盐向NAD的电子流。在充能状态下，与充能状态相比，抗充能蛋白II抗体对线粒体的抑制作用更大，这表明氧化还原反应伴随着充能蛋白II的构象变化。研究结果表明，URFA6L的产物chargerin II可能在线粒体氧化磷酸化的能量转导中起关键作用。
Antibody against chargerin II [product of the unidentified reading frame A6L (URFA6L) of mitochondrial DNA] inhibited the ATP-Pi exchange reaction and reversed electron flow from succinate to NAD in mitoplasts (inner membrane plus matrix). The antibody against chargerin II caused greater inhibition on incubation with mitoplasts in the energized than the nonenergized state, suggesting that redox reactions are coupled with a conformational change of chargerin II. The present findings showed that chargerin II, the URFA6L product, may have a key role in energy transduction of mitochondrial oxidative phosphorylation.