Differential role of peroxiredoxin II (PrxII) on the expression of toll-like receptor 4 (TLR4) and B-cell activating factor (BAFF) in ovalbumin (OVA)-induced mouse asthma
Differential role of peroxiredoxin II (PrxII) on the expression of toll-like receptor 4 (TLR4) and B-cell activating factor (BAFF) in ovalbumin (OVA)-induced mouse asthma
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DOI：
10.1016/j.intimp.2008.01.022
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发表时间：
2008-06-01
影响因子：
5.6
通讯作者：
Yu, Dae-Yeul
中科院分区：
文献类型：
--
作者：
Moon, Eun-Yi;Kang, Jong Soon;Yu, Dae-Yeul
过氧化物氧还蛋白II（Peroxiredoxin II，PrxII）是活性氧（reactive oxygen species，ROS）降解酶之一。在这里，我们研究了PrxII对Toll样受体4（TLR 4）和B细胞活化因子（BAFF）在卵清蛋白（OVA）诱导的小鼠哮喘中表达的作用。我们使用产生ROS的PrxII（-/-）小鼠，其细胞上调BAFF表达。由于实时定量RT-PCR分析检测到TLR 4 mRNA的显著变化，与野生型细胞相比，PrxII（-/-）小鼠脾细胞和腹腔巨噬细胞中的TLR 4蛋白减少。气道高反应性（AHR）在PrxII（-/-）小鼠中比野生型小鼠更严重，这通过各种参数的水平、嗜酸性粒细胞的数量、IgE水平、气道厚度和粘液分泌来测量。BAFF在OVA诱导的小鼠的气道周围细胞中检测到，并且在PrxII（-/-）小鼠中高度增强。BAFF启动子活性在PrxII（-/-）小鼠胚胎成纤维细胞（MEF）中也高于野生型MEF。总的来说，结果表明PrxII可能通过减少ROS而对哮喘有益。提示BAFF和TLR 4的表达受PrxII的不同调节，TLR 4的表达水平在OVA诱导的哮喘中可能不是决定性的。(C)2008年由Elsevier B. V.出版。
Peroxiredoxin II (PrxII) is one of reactive oxygen species (ROS)-degrading enzyme. Here, we investigated the role of PrxII on toll-like receptor 4 (TLR4) and B-cell activating factor (BAFF) expression in ovalbumin (OVA)-induced mouse asthma. We used ROS-producing PrxII(-/-) mice of which cells up-regulate BAFF expression. As significant changes were detected in TLR4 mRNA with real-time quantitative RT-PCR analysis, TLR4 protein was decreased in PrxII(-/-) mouse splenocytes and peritoneal macrophages, compared to wild type cells. Airway hyperresponsiveness (AHR) was more severe in PrxII(-/-) mice than wild type mice, which was measured by the level of various parameters, number of eosinophils, IgE level, airway thickness, and mucous secretion. BAFF was detected in cells surrounding airways of OVA-induced mouse and it was highly augmented in PrxII(-/-) mice. BAFF promoter activity was also higher in PrxII(-/-) mouse embryonic fibroblast (MEF) than in wild type MEF. Collectively, results show that PrxII may have benefits in asthma through reducing ROS. It suggests that BAFF and TLR4 expressions are differentially regulated by PrxII and TLR4 protein level may not be crucial in OVA-induced asthma. (C) 2008 Published by Elsevier B.V.