Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein
Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein
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DOI：
10.1080/22221751.2019.1685912
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发表时间：
2019-01-01
影响因子：
13.2
通讯作者：
Vergara-Alert, Julia
中科院分区：
文献类型：
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作者：
Rodon, Jordi;Okba, Nisreen M. A.;Vergara-Alert, Julia
持续的中东呼吸综合征冠状病毒（MERS-CoV）疫情对全球公共卫生构成威胁。阻断MERS-CoV人畜共患疾病从单峰骆驼（动物宿主）传播，可能会减少主要人类病例的数量。在这里，我们报告MERS-CoV从实验感染的美洲驼到幼稚动物的传播。直接接种的美洲驼排毒至少6天，并可在暴露后4-5天感染所有接触过的未感染动物。为了阻断病毒传播，我们研究了重组刺突S1蛋白疫苗的有效性。与未接种动物相反，接触接种的美洲驼在暴露于直接接种的美洲驼后不脱落感染性病毒，这与诱导强病毒中和抗体应答一致。我们的数据提供了进一步的证据，疫苗接种的水库主机可能会阻止MERS冠状病毒人畜共患病传播给人类。
The ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission from experimentally infected llamas to naive animals. Directly inoculated llamas shed virus for at least 6 days and could infect all in-contact naive animals 4-5 days after exposure. With the aim to block virus transmission, we examined the efficacy of a recombinant spike S1-protein vaccine. In contrast to naive animals, in-contact vaccinated llamas did not shed infectious virus upon exposure to directly inoculated llamas, consistent with the induction of strong virus neutralizing antibody responses. Our data provide further evidence that vaccination of the reservoir host may impede MERS-CoV zoonotic transmission to humans.