Caspases and matrix metalloproteases facilitate collective behavior of non-neural ectoderm after hindbrain neuropore closure.
Caspases and matrix metalloproteases facilitate collective behavior of non-neural ectoderm after hindbrain neuropore closure.
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胱天蛋白酶和基质金属蛋白酶有助于后脑神经孔闭合后非神经外胚层的集体行为。
DOI：
10.1186/s12861-018-0175-3
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发表时间：
2018-07-31
影响因子：
--
通讯作者：
Miura M
中科院分区：
文献类型：
--
作者：
Shinotsuka N;Yamaguchi Y;Nakazato K;Matsumoto Y;Mochizuki A;Miura M
哺乳动物脑通过神经管闭合（NTC）形成，其中相对的神经褶皱的两个脊在中线中融合并在神经管的顶板和覆盖非神经外胚层层中重塑。从NTC开始在神经嵴处广泛观察到细胞凋亡，并且对于NTC的适当进展至关重要，但其在关闭后的作用仍不清楚。在这里，我们对中脑神经孔（MHNP）闭合进行了实时成像分析，并揭示了MHNP周围细胞的意外集体行为。细胞首先聚集到闭合点，然后重新定位，就好像它们从该点释放一样。用化学抑制剂抑制半胱天冬酶或基质金属蛋白酶损害了细胞的重新定位。这些证据表明，骨化症介导的细胞外基质的降解可能有助于神经孔关闭的最终过程。本文的在线版本（10.1186/s12861-018-0175-3）包含补充材料，可供授权用户使用。
Mammalian brain is formed through neural tube closure (NTC), wherein both ridges of opposing neural folds are fused in the midline and remodeled in the roof plate of the neural tube and overlying non-neural ectodermal layer. Apoptosis is widely observed from the beginning of NTC at the neural ridges and is crucial for the proper progression of NTC, but its role after the closure remains less clear. Here, we conducted live-imaging analysis of the mid-hindbrain neuropore (MHNP) closure and revealed unexpected collective behavior of cells surrounding the MHNP. The cells first gathered to the closing point and subsequently relocated as if they were released from the point. Inhibition of caspases or matrix metalloproteases with chemical inhibitors impaired the cell relocation. These lines of evidence suggest that apoptosis-mediated degradation of extracellular matrix might facilitate the final process of neuropore closure. The online version of this article (10.1186/s12861-018-0175-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1083/jcb.201104057
发表时间：
2011-12-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yamaguchi Y;Shinotsuka N;Nonomura K;Takemoto K;Kuida K;Yosida H;Miura M
通讯作者：
Miura M
影响因子：
4.6
作者：
Cuervo, R;Covarrubias, L
通讯作者：
Covarrubias, L
影响因子：
64.5
作者：
Geisbrecht, ER;Montell, DJ
通讯作者：
Montell, DJ
DOI：
10.1016/j.cub.2015.12.072
发表时间：
2016-03-07
期刊：
Current biology : CB
影响因子：
--
作者：
Levayer R;Dupont C;Moreno E
通讯作者：
Moreno E
影响因子：
4.6
作者：
Gutzman, Jennifer H.;Sive, Hazel
通讯作者：
Sive, Hazel