COMPARISON OF A RADIOMETRIC METHOD (BACTEC) AND CONVENTIONAL CULTURE MEDIA FOR RECOVERY OF MYCOBACTERIA FROM SMEAR-NEGATIVE SPECIMENS
COMPARISON OF A RADIOMETRIC METHOD (BACTEC) AND CONVENTIONAL CULTURE MEDIA FOR RECOVERY OF MYCOBACTERIA FROM SMEAR-NEGATIVE SPECIMENS
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DOI：
10.1128/jcm.18.2.384-388.1983
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发表时间：
1983-01-01
影响因子：
9.4
通讯作者：
ROBERTS, GD
中科院分区：
文献类型：
--
作者：
MORGAN, MA;HORSTMEIER, CD;ROBERTS, GD
比较了 BACTEC 系统和 3 种常规培养基（Middlebrook 7H10、选择性 Middlebrook 7H11 [S7H11] 和 Lowenstein-Jensen [LJ]）的平均恢复时间和耐酸涂阴临床标本中分枝杆菌的恢复率。从 2165 份提交的涂片阴性培养物中回收的 71 份涂片阴性、培养物阳性标本中，BACTEC 系统检出率为 71.8%，而传统 3 培养基系统的检出率为 88.7%。当单独比较培养基时，BACTEC 培养基 (Middlebrook 7H12) 在回收分枝杆菌 (71.8%) 方面比 LJ (62%)、Middlebrook 培养基 7H10 (55.9%) 或 Middlebrook S7H11 培养基 (52.1%) 更成功。还对含有硒酸钠的 Middlebrook 7H11 培养基进行了评估，与 LJ、Middlebrook 7H10、S7H11 和 7H12 培养基相比，它没有提高分枝杆菌菌种的恢复率或缩短恢复时间。 BACTEC 系统对 7 种细菌的平均检测时间比传统方法短。分枝杆菌恢复。 BACTEC 系统和传统培养系统上结核分枝杆菌的检测时间分别为 13.7 天和 26.3 天。
The BACTEC system and 3 conventional media (Middlebrook 7H10, selective Middlebrook 7H11 [S7H11] and Lowenstein-Jensen [LJ]) were compared for their mean recovery times and recovery rates of mycobacteria from acid-fast, smear-negative clinical specimens. Of the 71 smear-negative, culture-positive specimens recovered from 2165 submitted smear-negative cultures, the BACTEC system detected 71.8%, compared with 88.7% for the conventional 3-medium system. When media were individually compared, BACTEC medium (Middlebrook 7H12) was more successful in recovering mycobacteria (71.8%) than was LJ (62%), Middlebrook medium 7H10 (55.9%) or Middlebrook S7H11 medium (52.1%). Middlebrook 7H11 medium containing sodium selenate was also evaluated and did not increase the recovery rate or decrease the recovery time of mycobacterial species when compared with LJ, Middlebrook 7H10, S7H11 and 7H12 media. The mean detection time for the BACTEC system was less than that by conventional methods for the 7 spp. of mycobacteria recovered. Detections times for Mycobacterium tuberculosis on the BACTEC system and conventional cultural systems were 13.7 and 26.3 days, respectively.