Porous Vesicles with Extrusion-Tunable Permeability and Pore Size from Mixed Solutions of PEO-PPO-PEO Triblock Copolymers
Porous Vesicles with Extrusion-Tunable Permeability and Pore Size from Mixed Solutions of PEO-PPO-PEO Triblock Copolymers
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PEO-PPO-PEO 三嵌段共聚物混合溶液中具有挤出可调渗透性和孔径的多孔囊泡
DOI：
10.1002/macp.201700620
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发表时间：
2018
影响因子：
2.5
通讯作者：
Kumar, Manish
中科院分区：
文献类型：
--
作者：
Schantz, A. Benjamin;Ren, Tingwei;Pachalla, Abhishek;Shen, Yuexiao;Hickey, Robert J.;Kumar, Manish
水溶液中的嵌段共聚物（BC）囊泡可以包封亲水性分子或纳米颗粒，用于药物和基因递送、增强成像、微反应器或传感器。廉价的、生物相容的聚（环氧乙烷）-聚（环氧丙烷）-聚（环氧乙烷）（PEO-PPO-PEO）三嵌段共聚物（TBC）对于这些应用将是理想的，如果它们可以形成浓缩的囊泡溶液以高效地包封这些分子的话。结果表明，两种PEO-PPO-PEO TBC（E0 5-P0 68-E0 5和E0 100-P0 65-E0 100）的溶液形成囊泡，囊泡膜可渗透低分子量（MW）溶质，挤出降低了膜渗透性和MW截留。挤出前后的渗透率分别为1000和1010 µm s−1，而溶质截留的MW截止值分别为1000和10400 g mol−1。因此，假设囊泡含有孔，并且挤出减小孔的尺寸和膜的孔隙率。这些选择性渗透的囊泡可以作为微反应器或传感器，封装大的溶质，而不需要添加通道分子或蛋白质。
Block copolymer (BC) vesicles in aqueous solution can encapsulate hydrophilic molecules or nanoparticles for drug and gene delivery, enhanced imaging, microreactors, or sensors. Inexpensive, biocompatible poly(ethylene oxide)–poly(propylene oxide)–poly(ethylene oxide) (PEO–PPO–PEO) triblock copolymers (TBCs) would be ideal for these applications if they could form concentrated vesicle solutions to encapsulate such molecules with high efficiency. It is shown that solutions of two PEO–PPO–PEO TBCs (EO5–PO68–EO5and EO100–PO65–EO100) form vesicles, that the vesicle membranes are permeable to low‐molecular weight (MW) solutes, and that extrusion reduces the membrane permeability and MW cutoff. Osmotic permeabilities before and after extrusion are ≈1000 and ≈10 µm s−1, respectively, while the MW cutoffs for solute rejection are ≈1000 and ≈400 g mol−1. Therefore, it is hypothesized that the vesicles contain pores, and that extrusion reduces the pores' size and the membrane's porosity. These selectively permeable vesicles can function as microreactors or sensors encapsulating large solutes without the need to add channel molecules or proteins.