An oviduct glycan increases sperm lifespan by diminishing the production of ubiquinone and reactive oxygen species†.
An oviduct glycan increases sperm lifespan by diminishing the production of ubiquinone and reactive oxygen species†.
复制标题
输卵管聚糖通过减少泛醌和活性氧的产生来延长精子的寿命。
DOI：
10.1093/biolre/ioad074
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发表时间：
2023
影响因子：
3.6
通讯作者：
Miller,DavidJ
中科院分区：
文献类型：
--
作者：
Hughes,JenniferR;McMorrow,KatieJ;Bovin,Nicolai;Miller,DavidJ
雌性动物在交配后储存精子的方法广泛应用于体内受精的动物，以使交配和排卵之间保持平衡。许多哺乳动物将精子储存在输卵管下部，输卵管上皮细胞上的特定聚糖保留精子以形成储库。与输卵管细胞结合抑制精子细胞内Ca 2+，增加精子寿命。我们研究了一种特殊的输卵管聚糖，3-O-硫酸化刘易斯X三糖（suLeX）延长猪精子寿命的机制。使用靶向代谢组学，我们发现与suLeX结合减少了4-羟基苯甲酸的丰度，4-羟基苯甲酸是泛醌（也称为辅酶Q）的前体，加入后30分钟。泛醌在电子传递链（ETC）中充当电子受体。3-O-硫酸化刘易斯X三糖也抑制富马酸盐的形成。柠檬酸循环的一个组成部分，富马酸是由琥珀酸辅酶Q还原酶，它采用泛醌，也被称为复合物II的ETC。一致的ETC的活性降低，有害的活性氧（ROS）的生产减少。精子在输卵管中的寿命延长可能是因为ROS产生受到抑制，因为高浓度的ROS对精子有毒性作用。
Sperm storage by females after mating for species-dependent periods is used widely among animals with internal fertilization to allow asynchrony between mating and ovulation. Many mammals store sperm in the lower oviduct where specific glycans on oviduct epithelial cells retain sperm to form a reservoir. Binding to oviduct cells suppresses sperm intracellular Ca2+and increases sperm longevity. We investigated the mechanisms by which a specific oviduct glycan, 3-O-sulfated Lewis X trisaccharide (suLeX), prolongs the lifespan of porcine sperm. Using targeted metabolomics, we found that binding to suLeXdiminishes the abundance of 4-hydroxybenzoic acid, the precursor to ubiquinone (also known as Coenzyme Q), 30 min after addition. Ubiquinone functions as an electron acceptor in the electron transport chain (ETC). 3-O-sulfated Lewis X trisaccharide also suppressed the formation of fumarate. A component of the citric acid cycle, fumarate is synthesized by succinate-coenzyme Q reductase, which employs ubiquinone and is also known as Complex II in the ETC. Consistent with the reduced activity of the ETC, the production of harmful reactive oxygen species (ROS) was diminished. The enhanced sperm lifespan in the oviduct may be because of suppressed ROS production because high ROS concentrations have toxic effects on sperm.