THE REPLICATIVE ORIGIN OF THE ESCHERICHIA-COLI CHROMOSOME BINDS TO CELL-MEMBRANES ONLY WHEN HEMIMETHYLATED
THE REPLICATIVE ORIGIN OF THE ESCHERICHIA-COLI CHROMOSOME BINDS TO CELL-MEMBRANES ONLY WHEN HEMIMETHYLATED
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DOI：
10.1016/0092-8674(88)90186-9
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发表时间：
1988-07-01
期刊：
影响因子：
64.5
通讯作者：
SCHAECHTER, M
中科院分区：
文献类型：
--
作者：
OGDEN, GB;PRATT, MJ;SCHAECHTER, M
来自大肠杆菌复制起点的 DNA 以高亲和力与外膜制剂结合。特异性结合区域包含在 oriC 图谱上位置 -46 和 +417 之间的 463 bp 起始 DNA 片段内。该 DNA 区域包含异常多的 GATC 位点，即大肠杆菌 DNA 腺嘌呤甲基化酶的识别序列。我们在此表明 oriC DNA 仅在半甲基化时才与膜结合。大肠杆菌染色体起点在复制开始后 8-10 分钟发生半甲基化，并且起点 DNA 仅在这段时间内与膜结合。基于这些结果，我们提出了大肠杆菌染色体分离的推测模型。
DNA from the E. coli replicative origin binds with high affinity to outer membrane preparations. Specific binding regions are contained within a 463 bp stretch of origin DNA between positions -46 and +417 on the oriC map. This region of DNA contains an unusually high number of GATC sites, the recognition sequence for the E. coli DNA adenine methylase. We show here that oriC DNA binds to membrane only when it is hemimethylated. The E. coli chromosomal origin is hemimethylated for 8-10 min after initiation of replication, and origin DNA binds to membranes only during this time period. Based on these results, we propose a speculative model for chromosome segregation in E. coli.