Two novel bacteriophages of thermophilic bacteria isolated from deep-sea hydrothermal fields
Two novel bacteriophages of thermophilic bacteria isolated from deep-sea hydrothermal fields
复制标题
从深海热液区分离出两种新型嗜热细菌噬菌体
DOI：
10.1007/s00284-005-0509-9
复制
发表时间：
2006-08-01
影响因子：
2.6
通讯作者：
Xie, Lianhui
中科院分区：
文献类型：
--
作者：
Liu, Bin;Wu, Suijie;Xie, Lianhui
嗜热噬菌体由于在许多地球化学和生态过程中的重要作用而引起人们的极大兴趣。然而，迄今为止还没有从深海嗜热细菌中分离出病毒体。本研究首次从太平洋深海热液区分离纯化了两种嗜热细菌裂解性噬菌体（命名为芽孢杆菌病毒W1和地芽孢杆菌病毒E1）。芽孢杆菌病毒W1（Bacillusvirus W1，BVW 1）是从芽孢杆菌（Bacillussp.w13）中分离得到的一种病毒，尾部长300 nm，宽15 nm，头部为六角形，直径70 nm。另一种病毒，来自Geobacillus sp. E26323的Geobacillus virus E1（GVE 1），是典型的Siphoviridae噬菌体，具有六边形的头部（直径130 nm）和尾部（长度180 nm，宽度30 nm）。这两个引物含有双链基因组DNA。BVW 1和GVE 1的基因组DNA大小估计分别为约18和41 kb。基于纯化的病毒粒子的SDS-PAGE，六个主要蛋白揭示了两种病毒中的每一种。本文的研究结果将有助于深入了解病毒对深海热液区嗜热菌及群落的影响。
Bacteriophages of thermophiles are of great interest due to their important roles in many biogeochemical and ecological processes. However, no virion has been isolated from deep-sea thermophilic bacteria to date. In this investigation, two lytic bacteriophages (termed Bacillus virus W1 and Geobacillus virus E1) of thermophilic bacteria were purified from deep-sea hydrothermal fields in the Pacific for the first time. Bacillus virus W1 (BVW1) obtained from Bacillus sp. w13, had a long tail (300nm in length and 15 nm in width) and a hexagonal head (70 nm in diameter). Another virus, Geobacillus virus E1 (GVE1) from Geobacillus sp. E26323, was a typical Siphoviridae phage with a hexagonal head (130 nm in diameter) and a tail (180 nm in length and 30 nm in width). The two phages contained double-stranded genomic DNAs. The genomic DNA sizes of BVW1 and GVE1 were estimated to be about 18 and 41 kb, respectively. Based on SDS-PAGE of purified virions, six major proteins were revealed for each of the two phages. The findings in our study will be very helpful to realize the effect of virus on thermophiles as well as the communities in deep-sea hydrothermal fields.