Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding
Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding
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通过比较染色体涂色、G 和 C 带揭示河马科（翼手目、哺乳动物）的核型进化
DOI：
10.3724/sp.j.1141.2010.05453
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发表时间：
2010-10-01
影响因子：
4.9
通讯作者：
Nie Wen-Hui
中科院分区：
文献类型：
--
作者：
Mao Xiu-Guang;Wang Jin-Huan;Nie Wen-Hui
与其姊妹科（菊头蝠科）相比，蹄蝠科的细胞遗传学研究较少。迄今为止，只有少数高分辨率的G-显带核型被报道，并且大多数关于蹄蝠科核型进化的结论都是基于常规的Giemsa染色。本研究采用比较染色体涂染技术（一种在分子水平上进行全基因组比较的方法），结合G-和C-分带技术，利用中国5种马蹄甲（Aselliscus stoliczkanus）的一整套染色体特异涂染探针，建立了中国马蹄甲科马蹄甲属通过对染色体彩绘所定义的同源片段进行G带和C带比较，发现罗伯逊易位、近着丝粒倒位和异染色质添加可能是蹄蝠科染色体进化的主要机制。通过对5种蹄蝠和外群种的保守染色体片段的比较分析，表明蹄蝠科的祖先核型中应该包括双臂染色体，而过去认为蹄蝠科的祖先核型完全由近端着丝粒染色体组成。
Comparing to its sister-family (Rhinolophidae), Hipposideridae was less studied by cytogenetic approaches. Only a few high-resolution G-banded karyotypes have been reported so far, and most of the conclusions on the karyotypic evolution in Hipposideridae were based on conventional Giemsa-staining. In this study, we applied comparative chromosome painting, a method of choice for genome-wide comparison at the molecular level, and G- and C-banding to establish comparative map between five hipposiderid species from China, using a whole set of chromosome-specific painting probes from one of them (Aselliscus stoliczkanus). G-band and C-band comparisons between homologous segments defined by chromosome painting revealed that Robertsonian translocations, paracentric inversions and heterochromatin addition could be the main mechanism of chromosome evolution in Hipposideridae. Comparative analysis of the conserved chromosomal segments among five hipposiderid species and outgroup species suggests that bi-armed chromosomes should be included into the ancestral karyotype of Hipposideridae, which was previously believed to be exclusively composed of acrocentric chromosomes.