The transcription factor interferon regulatory factor 1 (IRF-1) is important during the maturation of natural killer 1.1+ T cell receptor-alpha/beta+ (NK1+ T) cells, natural killer cells, and intestinal intraepithelial T cells.
The transcription factor interferon regulatory factor 1 (IRF-1) is important during the maturation of natural killer 1.1+ T cell receptor-alpha/beta+ (NK1+ T) cells, natural killer cells, and intestinal intraepithelial T cells.
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DOI：
10.1084/jem.187.6.967
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发表时间：
1998-03-16
期刊：
影响因子：
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通讯作者：
Ohashi PS
中科院分区：
文献类型：
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作者：
Ohteki T;Yoshida H;Matsuyama T;Duncan GS;Mak TW;Ohashi PS
与传统T细胞不同，自然杀伤细胞(NK)1.1+T细胞受体(TCRNK1+T)、NK细胞和带有α/β-α/α链的肠上皮内淋巴细胞(IEL)构成表达白细胞介素2受体(IL)-2受体(R)β/15Rβ链。最近的研究表明，IL-2Rβ/15Rβ链是这些淋巴细胞亚群发育所必需的，概述了IL-15的重要性。在这项研究中，我们研究了这些淋巴细胞亚群在干扰素调节因子1缺陷(−/−)小鼠中的发育情况。令人惊讶的是，在irf-1−/−小鼠中，所有这些淋巴细胞亚群都严重减少。在CD8-α/α+肠道IEL亚群中，TcR-γ/δ+细胞和TcR-α/β+细胞同样受到IRF基因突变的影响。与肠道TcR-γ/δ+细胞相比，胸腺Tcr-γ/δ+细胞在IRF-1−/−小鼠体内发育正常。Northern印迹分析进一步表明，−/−细胞IL-15信使核糖核酸的诱导受到抑制，IL-15与−/−细胞共同培养可观察到这些淋巴细胞亚群的恢复。提示IRF-1调节IL-15基因表达，可能控制NK1+T细胞、NK细胞和CD8-α/α+IEL的发育。
In contrast to conventional T cells, natural killer (NK) 1.1+ T cell receptor (TCR)-α/β+ (NK1+T) cells, NK cells, and intestinal intraepithelial lymphocytes (IELs) bearing CD8-α/α chains constitutively express the interleukin (IL)-2 receptor (R)β/15Rβ chain. Recent studies have indicated that IL-2Rβ/15Rβ chain is required for the development of these lymphocyte subsets, outlining the importance of IL-15. In this study, we investigated the development of these lymphocyte subsets in interferon regulatory factor 1–deficient (IRF-1−/−) mice. Surprisingly, all of these lymphocyte subsets were severely reduced in IRF-1−/− mice. Within CD8-α/α+ intestinal IEL subset, TCR-γ/δ+ cells and TCR-α/β+ cells were equally affected by IRF gene disruption. In contrast to intestinal TCR-γ/δ+ cells, thymic TCR-γ/δ+ cells developed normally in IRF-1−/− mice. Northern blot analysis further revealed that the induction of IL-15 messenger RNA was impaired in IRF-1−/− bone marrow cells, and the recovery of these lymphocyte subsets was observed when IRF-1−/− cells were cultured with IL-15 in vitro. These data indicate that IRF-1 regulates IL-15 gene expression, which may control the development of NK1+T cells, NK cells, and CD8-α/α+ IELs.