Cell permeabilization using a non-thermal plasma
Cell permeabilization using a non-thermal plasma
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DOI：
10.1088/1367-2630/11/11/115021
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发表时间：
2009-11-26
影响因子：
3.3
通讯作者：
Coulombe, S.
中科院分区：
文献类型：
--
作者：
Leduc, M.;Guay, D.;Coulombe, S.
大气压辉光放电炬（APGD-t）是一种微型脉冲RF（13.56 MHz）等离子体源，专门设计用于有效地产生和传输反应性物质，并与生物样品相互作用。当应用于贴壁细胞培养时，APGD-t能够诱导暂时的细胞透化。在这项工作中，我们评估的最大半径的大分子能够进入HeLa细胞后，使用葡聚糖分子的尺寸排阻方法的血浆治疗。我们观察到这个最大半径低于6.5 nm。我们还表明，没有降解发生时，质粒DNA悬浮在培养基中的等离子体处理的操作条件下，导致细胞透化。证明了使用APGD-t的具有hrGFP-II-1质粒的HeLa细胞的等离子体辅助的DNA转染。获得的局部转染效率高达35%。
The atmospheric pressure glow discharge torch (APGD-t) is a miniature pulsed RF (13.56 MHz) plasma source specifically designed to efficiently produce and transport reactive species to and interact with biological samples. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. We observe that this maximum radius is below 6.5 nm. We also show that no degradation occurs when a plasmid DNA suspended in culture media is plasma-treated at the operating conditions leading to cell permeabilization. The plasma-assisted DNA transfection of the HeLa cells with the hrGFP-II-1 plasmid using the APGD-t is demonstrated. Local transfection efficiencies obtained are as high as 35%.