Comparison of gargle samples and throat swab samples for the detection of respiratory pathogens
Comparison of gargle samples and throat swab samples for the detection of respiratory pathogens
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DOI：
10.1016/j.jviromet.2017.06.010
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发表时间：
2017-10-01
影响因子：
3.1
通讯作者：
Gunson, Rory N.
中科院分区：
文献类型：
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作者：
Bennett, Susan;Davidson, Rhona S.;Gunson, Rory N.
呼吸道疾病导致严重的发病率，特别是在儿童、老年人和免疫功能低下者中。采集的样本类型和样本质量对于提供准确的诊断具有重要意义。漱口样本很容易采取和采样相同的区域作为咽喉拭子（THS）。在这项研究中，我们评估了漱口样本的实用性，常见的呼吸道感染的分子检测。比较同一患者当天采集的配对漱口液和THS样本。我们的分析还包括在三天内收集的配对THS和漱口液样本，因为这些样本可能是在同一疾病期间采集的。总体而言，数据表明漱口样品是比THS样品更敏感的样品类型，因为总体而言，漱口样品的诊断率更高，并且与THS样品相比，大多数样品的漱口样品的Ct值更强。在1 - 3天内收集的配对样本中观察到相似的数据，因为尽管样本类型之间的诊断率相似（相似的不一致结果），但大多数漱口液的Ct值比THS样本更强。本文强调了漱口液样品作为非侵入性敏感呼吸样品与THS样品相比的有用性。我们建议其他检测中心应考虑使用漱口液样本进行呼吸道诊断，因为这将在灵敏度和采样易用性方面带来好处。
Respiratory illness causes significant morbidity especially in children, the elderly and the immunocompromised. The sample type taken and the quality of that sample are of great significance in providing an accurate diagnosis. Gargle samples are easy to take and sample the same area as a throat swab (THS). In this study, we assessed the utility of gargle samples for the molecular detection of common respiratory infections. Paired gargle and THS samples collected on the same day from the same patient were compared. We also included in our analysis paired THS and gargle samples that were collected within three days of each other as these samples are likely to have been taken during the same illness. Overall the data suggests that gargle samples are a more sensitive sample type than THS samples as overall the diagnostic yield was higher in the gargle samples and the Ct value of the gargle samples was stronger for the majority of samples in comparison to THS samples. Similar data was seen in the paired samples collected within one to three days of each other, as although the diagnostic yield between the sample types was similar (similar discrepant results), the majority of gargles had stronger Ct values than THS samples. This paper highlights the usefulness of gargle samples as non-invasive sensitive respiratory sample in comparison to THS samples. We recommend that other testing sites should consider using gargle samples for respiratory diagnosis as it will bring benefits in terms of sensitivity and sampling ease of use.