Expression and regulation of Smad2 by gonadotropins in the protogynous hermaphroditic ricefield eel (Monopterus albus)
Expression and regulation of Smad2 by gonadotropins in the protogynous hermaphroditic ricefield eel (Monopterus albus)
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DOI：
10.1007/s10695-020-00778-9
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发表时间：
2020-03-03
影响因子：
2.9
通讯作者：
Yan, Taiming
中科院分区：
文献类型：
--
作者：
He, Zhi;Deng, Faqiang;Yan, Taiming
Smad2 是一种受体激活的 Smad，在调节配子发生中发挥着关键作用。本研究从稻田鳗卵巢 cDNA 中鉴定并测序了 smad2 同源物，并分析了卵母细胞发育过程中其 mRNA 和蛋白表达水平。稻田鳗smad2的cDNA序列由1863 bp组成，编码467个氨基酸的蛋白质，与其他硬骨鱼中的Smad蛋白具有高度的序列同源性，特别是多产Poeciliopsis prolifica中的Smad蛋白。实时定量PCR（RT-qPCR）分析结果显示，smad2在性腺发育过程中在卵巢中表达，并持续增加直至卵巢卵黄发生早期，然后随着卵巢成熟而减少。 Smad2 蛋白免疫反应性位于滤泡细胞、卵原细胞和初级生长期卵母细胞的细胞质中。体外实验表明，卵泡刺激素（FSH）和人绒毛膜促性腺激素（hCG）分别以时间和剂量依赖性方式促进卵巢组织中smad2的表达。综上所述，Smad2在稻田鳗卵巢发育中发挥着潜在的重要作用。
Smad2, a receptor-activated Smad, plays a critical role in regulating gametogenesis. In this study, a smad2 homologue was identified and sequenced from ricefield eel ovary cDNA, and its mRNA and protein expression levels were analysed during oocyte development. The cDNA sequence of ricefield eel smad2 consisted of 1863 bp encoding a 467-amino acid protein that had high sequence homology with Smad proteins in other teleosts, especially in Poeciliopsis prolifica. The results of real-time quantitative PCR (RT-qPCR) analysis revealed that smad2 is expressed in the ovary during gonad development, increased continuously until the early vitellogenic stage in the ovaries, and then decreased with ovary maturation. Smad2 protein immunoreactivity was localized in the cytoplasm of follicular cells, oogonia, and primary growth stage oocytes. In vitro experiments revealed that follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG) promoted smad2 expression in ovary tissue in a time- and dose-dependent manner, respectively. In summary, Smad2 plays a potentially vital role in ricefield eel ovary development.