Platelet microparticles and soluble P selectin in peripheral artery disease: Relationship to extent of disease and platelet activation markers
Platelet microparticles and soluble P selectin in peripheral artery disease: Relationship to extent of disease and platelet activation markers
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DOI：
10.1080/07853890410018943
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发表时间：
2005-01-01
影响因子：
4.4
通讯作者：
Lip, GYH
中科院分区：
文献类型：
--
作者：
Tan, KT;Tayebjee, MH;Lip, GYH
背景资料。在许多心血管疾病中，存在着血小板活化增加。这一观察结果可能解释了这些疾病中存在的血小板微粒(PMP)水平升高的原因。然而，PMPs水平是否与其他血小板活化标志物，如可溶性P-选择素，或疾病严重程度相互关联尚不清楚。因此，我们假设稳定性外周动脉疾病(PAD)间歇性跛行(IC)患者的PMP水平升高，并伴有严重PAD临界肢体缺血(CLI)的额外增加。此外，我们还验证了PMP水平与其他血小板活化标志物如可溶性P-选择素、膜结合P-选择素(CD62P)和CD63相关的假设。PAD患者来自一家教学医院的血管门诊和住院设施。年龄和性别匹配的对照组也是从健康志愿者中招募的。取23例重症患者(CLI)、36例中度疾病(IC)患者和30例健康体检者的静脉血。用流式细胞仪检测CD62p、CD63阳性的血小板百分率和PMPs的数量。采用酶联免疫吸附试验(ELISA法)测定血浆sP选择素水平。IC患者PMPs较正常对照组升高，CLI进一步升高(P
Background. There is increased platelet activation in many cardiovascular diseases. This observation may explain the presence of increased levels of platelet microparticles (PMP) in these diseases. However, whether or not levels of PMPs inter-relate with other markers of platelet activation, such as soluble P-selectin, or with disease severity, is unknown. We therefore hypothesized raised PMP levels in stable peripheral artery disease (PAD) intermittent claudication (IC), with an additional increase in severe PAD critical limb ischaemia (CLI). Furthermore, we tested the hypothesis that PMP levels are correlated with other markers of platelet activation, such as soluble P-selectin, membrane bound P-selectin (CD62P) and CD63.Methods. Patients with PAD were recruited from the vascular outpatient and inpatient facilities at a teaching hospital. Age and sex-matched controls were also recruited from healthy volunteers. Venous blood was obtained from 23 patients with severe disease (CLI), 36 with moderate disease (IC), and from 30 healthy controls. The percentage of platelets positive for CD62P and CD63, as well as the numbers of PMPs were defined by flow cytometry. Plasma soluble P selectin was measured by enzyme-linked immunosorbent assay (ELISA).Results. PMPs were increased relative to healthy controls in patients with IC, with a further increase in CLI (P