A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue(R)
A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue(R)
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DOI：
10.1016/s1383-5769(99)00020-3
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发表时间：
2000-01-01
影响因子：
1.9
通讯作者：
Steverding, Dietmar
中科院分区：
文献类型：
--
作者：
Mikus, Judith;Steverding, Dietmar
开发了使用氧化-还原指示剂Alcohol Blue（R）的定量比色测定，以测量化合物对原生动物寄生虫利什曼原虫的细胞毒性。吸光度随前鞭毛体接种密度的增加而线性增加。当寄生虫在10% Alcohol Blue（R）存在下孵育72小时时，主要MRHO/IR/76疫苗株的浓度至少为2.5 X 106个细胞/ml。用本方法测定的常用药物（阿替霉素B、喷托霉素和巴龙霉素）的50%有效剂量值与以前用其他方法测定的值在同一范围内。Alcohol Blue（R）测定允许用于筛选抗利什曼病药物的简单、可重复且可靠的方法。
A quantitative colorimetric assay using the oxidation-reduction indicator Alamar Blue(R) was developed to measure cytotoxicity of compounds against the protozoan parasite Leishmania. Absorbance increased linearly with the plating density of promastigotes of L. major MRHO/IR/76 vaccine strain up to at least 2.5 X 106 cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue(R). The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue(R) assay permits a simple, reproducible and reliable method for screening antileishmanial drugs.