A multistage testing strategy for detection of quantitative trait loci affecting disease resistance in Atlantic salmon
A multistage testing strategy for detection of quantitative trait loci affecting disease resistance in Atlantic salmon
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DOI：
10.1534/genetics.103.013227
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发表时间：
2004-06-01
期刊：
影响因子：
3.3
通讯作者：
Gomez-Raya, L
中科院分区：
文献类型：
--
作者：
Moen, T;Fjalestad, KT;Gomez-Raya, L
提出了一种多阶段检测策略，以检测大西洋鲑鱼抗感染性鲑鱼贫血（伊萨）的QTL。首先，使用抗病性激发试验的死亡后代进行扩增片段长度多态性（AFLP）的基因分型和传递不平衡检验（TDT）。第二，AFLP基因分型的幸存者随后进行孟德尔分离测试。第三，使用所有后代的家庭内生存分析，并适用于显着的TDT标记与孟德尔遗传。最大似然法开发TDT与显性标记，利用家庭内的连锁不平衡。该策略在由同一雄性繁殖的两个大西洋鲑鱼全同胞家族中进行了测试，总共由79只后代组成。所有死亡的后代从两个家庭进行了分型的64个引物组合，导致340评分标记。在401例TDT中，有26例结果显著。在第二阶段，只有17个标记家系表现出孟德尔分离，并进行了生存分析。对所有生存分析进行排列检验，以计算实验P值。两个市场，aaccac356和agccta150，在P < 0.05时，在生存分析中解释多重检验时是显著的。所提出的策略可能比当前的映射策略更强大，因为它减少了在最后测试阶段要执行的测试的数量。
A multistage testing strategy, to detect QTL for resistance to infections salmon anemia (ISA) in Atlantic salmon is proposed. First, genotyping of amplified fragment length polymorphisms (AFLP) and a transmission disequilibrium test (TDT) were carried out using dead offspring from a disease resistance challenge test. Second, AFLP genotyping among survivors followed by a Mendelian segregation test was performed. Third, within-family survival analyses using all offspring were developed and applied to significant TDT markers with Mendelian inheritance. Maximum-likelihood methodology was developed for TDT with dominant markers to exploit linkage disequilibrium within families. The strategy was tested with two full-sib families of Atlantic salmon sired by the same male and consisting of 79 offspring in total. All dead offspring from the two families were typed for 64 primer combinations, resulting in 340 scored markers. There were 26 significant results out Of 401 TDTs using (lead offspring. In the second stage, only 17 marker families showed Mendelian segregation and were tested in survival analysis. A permutation test was performed for all survival analyses to compute experimentwise P-values. Two markets, aaccac356 and agccta150, were significant at P < 0.05 when accounting for nuiltiple testing in the survival analyses. The proposed strategy might be more powerful than current mapping strategies because it. reduces the number of tests to be performed in the last testing stage.