Three-Dimensional Microscopy Demonstrates Series and Parallel Organization of Human Peripapillary Capillary Plexuses
Three-Dimensional Microscopy Demonstrates Series and Parallel Organization of Human Peripapillary Capillary Plexuses
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DOI：
10.1167/iovs.18-24105
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发表时间：
2018-09-01
影响因子：
4.4
通讯作者：
Balaratnasingam, Chandrakumar
中科院分区：
文献类型：
--
作者：
Chandrasekera, Erandi;An, Dong;Balaratnasingam, Chandrakumar
目的。定义人体视乳头周围毛细血管丛的三维拓扑组织，以便更好地了解视网膜循环的血流动力学特征。在五只正常人供体眼睛中对视网膜微脉管系统进行灌注标记，并使用共焦扫描激光显微镜从视盘的上、颞、下和鼻区域收集光学堆栈。比较毛细血管丛的空间位置和形态特征。三维可视化策略用于记录互连毛细血管床的血管导管的组织，并研究毛细血管床与毛细血管前小动脉和毛细血管后小静脉之间的通信。结果。视乳头周围微循环由4个毛细血管丛组成，包括神经纤维层水平的径向毛细血管丛、神经节细胞层水平的浅毛细血管丛（SCP）、位于内核层内边界的中间毛细血管丛和位于内核层外边界的深部毛细血管丛。四个丛之间的毛细血管直径和密度有显着差异（均为 P
PURPOSE. To define the three-dimensional topologic organization of the human peripapillary capillary plexuses in order to better understand the hemodynamic characteristics of this retinal circulation.METHODS. The retinal microvasculature was perfusion labeled in five normal human donor eyes, and optical stacks were collected from regions immediately superior, temporal, inferior, and nasal to the optic disk by using confocal scanning laser microscopy. The spatial location and morphometric characteristics of capillary plexuses were compared. Three-dimensional visualization strategies were used to document the organization of vascular conduits that interconnect capillary beds and to study the communications between capillary beds and precapillary arterioles and postcapillary venules.RESULTS. The peripapillary microcirculation is composed of four capillary plexuses, including the radial peripapillary capillary plexus at the level of the nerve fiber layer, the superficial capillary plexus (SCP) at the level of the ganglion cell layer, the intermediate capillary plexus located at the inner boundary of the inner nuclear layer, and the deep capillary plexus located at the outer boundary of the inner nuclear layer. Capillary diameter and density were significantly different between the four plexuses (both P