CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis
CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis
复制标题
CircRNA hsa_circ_0004771 通过 miR-339-5p/CDC25A 轴促进食管鳞状细胞癌进展。
DOI：
10.2217/epi-2019-0404
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发表时间：
2020-04-01
期刊：
影响因子：
3.8
通讯作者：
Li, Ke
中科院分区：
文献类型：
--
作者：
Huang, Enmin;Fu, Junhui;Li, Ke
目的：circRNA在食管鳞状细胞癌（ESCC）中的作用尚不清楚。材料和方法：在此，我们基于RNA测序分析了ESCC中的6对血浆circRNA，然后通过定量逆转录酶PCR验证了20个癌组织和105对病例对照血浆样本中hsa_circ_0004771的升高。结果如下：hsa_circ_0004771的表达上调与肿瘤负荷增加和预后不良相关，hsa_circ_0004771的表达下调在体内外均抑制食管鳞癌细胞的增殖。hsa_circ_0004771通过充当miR-339- 5 p的分子海绵正向调节CDC 25 A，并且拯救试验证实了这种调节关系。结论：这些结果表明hsa_circ_0004771可以作为一个通用的低侵袭性生物标志物，并可能提供诊断和预后价值的癌症。
Aim: The role of circRNAs in esophageal squamous cell cancer (ESCC) remains unclear. Materials & methods: Here we profiled six pair plasma circRNA in ESCC based on RNA sequencing, and then verified the elevation of hsa_circ_0004771 in 20 cancer tissues and 105 pair case-control plasma samples by quantitative reverse transcriptase PCR. Results: The upregulation of hsa_circ_0004771 was correlated with heavier tumor burden and poor prognosis, knockdown of it inhibited the ESCC cells proliferation both in vitro and in vivo. Mechanistically, hsa_circ_0004771 positively regulated CDC25A by acting as a molecular sponge of miR-339-5p and rescue assay confirmed this regulatory relationship. Conclusion: These results suggested that hsa_circ_0004771 can serve as a general less-invasive biomarker and may provide diagnostic and prognostic value in carcinoma.