Generation of tumor-specific transplantation antigens by UV radiation can occur independently of neoplastic transformation.
Generation of tumor-specific transplantation antigens by UV radiation can occur independently of neoplastic transformation.
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DOI：
10.4049/jimmunol.137.8.2721
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发表时间：
1986-10
影响因子：
4.4
通讯作者：
L. W. Hostetler;H. Ananthaswamy;M. Kripke
中科院分区：
文献类型：
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作者：
L. W. Hostetler;H. Ananthaswamy;M. Kripke
本研究的目的是确定是否紫外线相关抗原在小鼠诱导的肿瘤慢性紫外线照射可以诱导体外照射的细胞已经致瘤，或其发生是否与原发性肿瘤转化事件。将非抗原性的自发纤维肉瘤细胞系暴露于体外UV辐射，进行克隆，并测试抗原特性。大量的纤维肉瘤细胞的紫外线照射后获得的克隆是高度抗原性的（20 39），而来自未照射的培养物的克隆没有（0 10）。抗原性变体本身不诱导交叉保护，但在体内仅诱导变体特异性免疫。几种抗原变异体进行了测试，紫外线诱导的抑制细胞，这似乎承认一个共同的决定因素之间共享的紫外线诱导的肿瘤的行动的敏感性。测试的变体确实受到UV诱导的抑制性淋巴细胞的活性的影响。这些结果表明，紫外线诱导的小鼠皮肤癌所表现出的独特的抗原特性也表现出体外暴露于紫外线辐射的细胞。此外，它们意味着UV相关抗原是由于将细胞暴露于UV辐射而产生的，并且它们可以独立于初始肿瘤转化事件而发生。
The purpose of this study was to determine whether the UV-associated antigens present on tumors induced in mice by chronic UV irradiation could be induced by in vitro irradiation of cells that were already tumorigenic, or whether their occurrence was associated with the primary neoplastic transformation event. Cells of a nonantigenic, spontaneous fibrosarcoma cell line were exposed to UV radiation in vitro, were cloned, and were tested for antigenic properties. A large number of the clones obtained after UV irradiation of the fibrosarcoma cells were highly antigenic (20 of 39), whereas clones derived from unirradiated cultures were not (0 of 10). The antigenic variants did not induce cross-protection among themselves, but induced only variant-specific immunity in vivo. Several antigenic variants were tested for susceptibility to the action of UV-induced suppressor cells, which seem to recognize a common determinant shared among UV-induced tumors. The variants tested were indeed subject to the activity of the UV-induced suppressor lymphocytes. These results demonstrate that the unique antigenic properties exhibited by UV-induced murine skin cancers are also exhibited by cells exposed to UV radiation in vitro. In addition, they imply that the UV-associated antigens arise as a consequence of exposing cells to UV radiation and that they can occur independently of an initial neoplastic transformation event.