Efferent projections of the suprachiasmatic nucleus based on the distribution of vasoactive intestinal peptide (VIP) and arginine vasopressin (AVP) immunoreactive fibers in the hypothalamus of Sapajus apella
Efferent projections of the suprachiasmatic nucleus based on the distribution of vasoactive intestinal peptide (VIP) and arginine vasopressin (AVP) immunoreactive fibers in the hypothalamus of Sapajus apella
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DOI：
10.1016/j.jchemneu.2014.03.004
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发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Nogueira, M. I.
中科院分区：
文献类型：
--
作者：
Campos, L. M. G.;Cruz-Rizzolo, R. J.;Nogueira, M. I.
视交叉上核（SCN）被认为是哺乳动物的主生物钟，它建立了几个器官所表现出的大约24小时的生物节律。与生物节律性的神经功能特征的研究相关的一个方面是识别SCN和其他脑区之间的通信通路。因此，SCN传出投射已在几个物种，包括啮齿动物和一些灵长类动物进行了研究。来自两个主要内在纤维亚群的纤维，一个产生血管活性肠肽（VIP），另一个产生精氨酸加压素（AVP），表现出形态学特征，将它们与来自其他脑区的纤维区分开来。这种区别提供了一个参数来研究SCN传出投射。本研究采用免疫组织化学和形态学方法，对灵长类无鳍沙鱼（Sapajus apella）下丘脑内VIP（VIP-ir）和AVP（AVP-ir）免疫反应（ir）纤维和终末进行了定位。关于纤维分布模式，AVP-IR和VIP-IR纤维被确定在结节下丘脑区、视交叉后区、外侧下丘脑区和前下丘脑区的区域中。VIP-ir和AVP-ir纤维在下丘脑的几个区域共存，但在下丘脑后部和内侧室周区，AVP-ir纤维的优势比VIP-ir纤维。这种分布模式和接收下丘脑区的VIP-IR和AVP-IR纤维，其中共享类似的形态特征与SCN中发现的，是类似的模式中观察到的昼夜动物。这一发现支持了这一特征在不同物种中的保守性。SCN内源性神经元的形态计量学分析表明，在SCN腹侧部分的VIP-IR神经元的大小的同质性和在SCN背侧部分的AVP-IR神经元的两个亚群的大小的异质性。这些神经元群体的纤维和形态特征的分布进行了描述，并在本研究中与其他物种进行了比较。(C)2014爱思唯尔有限公司版权所有。
The suprachiasmatic nucleus (SCN), which is considered to be the master circadian clock in mammals, establishes biological rhythms of approximately 24 h that several organs exhibit. One aspect relevant to the study of the neurofunctional features of biological rhythmicity is the identification of communication pathways between the SCN and other brain areas. As a result, SCN efferent projections have been investigated in several species, including rodents and a few primates. The fibers originating from the two main intrinsic fiber subpopulations, one producing vasoactive intestinal peptide (VIP) and the other producing arginine vasopressin (AVP), exhibit morphological traits that distinguish them from fibers that originate from other brain areas. This distinction provides a parameter to study SCN efferent projections. In this study, we mapped VIP (VIP-ir) and AVP (AVP-ir) immunoreactive (ir) fibers and endings in the hypothalamus of the primate Sapajus apella via immunohistochemical and morphologic study. Regarding the fiber distribution pattern, AVP-ir and VIP-ir fibers were identified in regions of the tuberal hypothalamic area, retrochiasmatic area, lateral hypothalamic area, and anterior hypothalamic area. VIP-ir and AVP-ir fibers coexisted in several hypothalamic areas; however, AVP-ir fibers were predominant over VIP-ir fibers in the posterior hypothalamus and medial periventricular area. This distribution pattern and the receiving hypothalamic areas of the VIP-ir and AVP-ir fibers, which shared similar morphological features with those found in SCN, were similar to the patterns observed in diurnal and nocturnal animals. This finding supports the conservative nature of this feature among different species. Morphometric analysis of SCN intrinsic neurons indicated homogeneity in the size of VIP-ir neurons in the SCN ventral portion and heterogeneity in the size of two subpopulations of AVP-ir neurons in the SCN dorsal portion. The distribution of fibers and morphometric features of these neuronal populations are described and compared with those of other species in the present study. (C) 2014 Elsevier B.V. All rights reserved.