Application of the split root technique to study iron uptake in cucumber plants
Application of the split root technique to study iron uptake in cucumber plants
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DOI：
10.1016/j.plaphy.2012.05.022
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发表时间：
2012-08-01
影响因子：
6.5
通讯作者：
Zocchi, Graziano
中科院分区：
文献类型：
--
作者：
De Nisi, Patrizia;Vigani, Gianpiero;Zocchi, Graziano
从生化和分子水平研究了植物体内铁营养状况对黄瓜根系缺铁反应的调控作用。除了与铁缺乏反应严格相关的两种活性，即Fe（III）螯合还原酶和高亲和力铁转运蛋白的活性外，我们还考虑了H+-ATP酶（EC 3.6.3.6）和磷酸烯醇式丙酮酸羧化酶（EC 4.1.1.31），它们已被证明参与这种反应。酶的活性和基因表达进行了监测，使用分根系统。缺铁诱导的表达的四个成绩单，伴随着相应的酶活性的增加。裂根技术的应用提供了有关铁吸收调节的一些信息。事实上，24小时后，分根应用程序，成绩单仍然很高，可比的Fe控制在Fe供应的一半，而在Fe侧有一个下降的表达和相对酶活性。主要变化发生在48和72 h后。这些反应的协调调节进行了讨论。(C)2012年Elsevier Masson SAS。All rights reserved.
The regulation exerted by the Fe status in the plant on Fe deficiency responses was investigated in Cucumis sativus L roots at both biochemical and molecular levels. Besides the two activities strictly correlated with Fe deficiency response, those of the Fe(III) chelate reductase and the high affinity Fe transporter, we considered also H+-ATPase (EC 3.6.3.6) and phosphoenolpyruvate carboxylase (EC 4.1.1.31), that have been shown to be involved in this response. Both enzymatic activities and gene expression were monitored using a split root system. Absence of Fe induced the expression of the four transcripts, accompanied by an increase in the corresponding enzymatic activities. The application of the split root technique gave some information about the regulation of Fe uptake. In fact, 24 h after split root application, transcripts were still high and comparable to those of the -Fe control in the Fe-supplied half side, while in the -Fe side there was a drop in the expression and the relative enzymatic activities. Major changes occurred after 48 and 72 h. The coordinated regulation of these responses is discussed. (C) 2012 Elsevier Masson SAS. All rights reserved.