BANDED MARKER CHROMOSOMES AS INDICATORS OF INTRASPECIES CELLULAR CONTAMINATION
BANDED MARKER CHROMOSOMES AS INDICATORS OF INTRASPECIES CELLULAR CONTAMINATION
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DOI：
10.1126/science.184.4141.1093
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发表时间：
1974-01-01
期刊：
影响因子：
56.9
通讯作者：
HAWTHORN.PK
中科院分区：
文献类型：
--
作者：
NELSONRE.WA;FLANDERM.RR;HAWTHORN.PK
染色体显带显示了命名为HEK、HEK/HRV、HBT-3、HBT-39 B、MA 160和SA-4 TxS-HuSa 1菌株的培养物中HeLa细胞的标记染色体特征。发现的其他HeLa细胞特征是葡萄糖-6-磷酸脱氢酶A型流动性和缺乏Y染色体。常规染色体分析和免疫学及酶技术用于监测供体的种属特异性和种族来源。然而，染色体显带可以监测线性内核型特性及其在长期培养过程中的演变。
Chromosome banding revealed marker chromosomes characteristic of HeLa cells in cultures designated HEK, HEK/HRV, HBT-3, HBT-39B, MA160, and a strain of SA-4TxS-HuSa1. Other HeLa cell characteristics found were glucose-6-phosphate dehydrogenase type A mobility and lack of the Y chromosome. Conventional chromosome analysis and immunological and enzymatic techniques serve to monitor species specificity and racial origin of the donor. Chromosome banding, however, can monitor intralinear karyotype peculiarity and its evolution during long-term cultivation.