Myosin at the apical pole of ciliated epithelial cells as revealed by a monoclonal antibody.
Myosin at the apical pole of ciliated epithelial cells as revealed by a monoclonal antibody.
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单克隆抗体揭示的纤毛上皮细胞顶端的肌球蛋白。
DOI：
10.1083/jcb.103.2.613
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发表时间：
1986-08
期刊：
影响因子：
--
通讯作者：
Bornens M
中科院分区：
文献类型：
--
作者：
Klotz C;Bordes N;Laine MC;Sandoz D;Bornens M
在鹌鹑输卵管基底体作为免疫原的融合实验中获得了一种单克隆抗体（CC-212），该抗体已被证明可以标记纤毛细胞的顶极，并与200-kD蛋白反应。使用以下标准证明该单克隆抗体是来自平滑肌或非肌肉细胞的抗肌球蛋白：在Western blots上，它与来自砂囊和血小板提取物以及培养细胞系提取物的肌球蛋白重链反应，但与横纹肌肌球蛋白重链不反应。通过免疫荧光，它可以使扩散良好的细胞的应力纤维具有特征性的条纹图案，而不与含有有组织的肌原纤维的肌管发生反应。在天然纤毛细胞和triton提取的鹌鹑输卵管纤毛皮层上，该单克隆抗体在根尖极上具有较强的标记性。利用免疫金技术对同一制剂进行超微结构定位。肌球蛋白与一种丝状物质有关，这种丝状物质存在于有条纹的小根和基部的近端之间。没有观察到基底体本身或轴突的标记。
A monoclonal antibody (CC-212), obtained in a fusion experiment in which basal bodies from quail oviduct were used as immunogen, has been shown to label the apical pole of ciliated cells and to react with a 200-kD protein. This monoclonal antibody was demonstrated to be an anti- myosin from smooth muscle or from nonmuscular cells using the following criteria: On Western blots it reacted with the myosin heavy chains from gizzard and platelet extracts and from cultured cell line extracts, but did not react with striated muscle myosin heavy chains. By immunofluorescence it decorated the stress fibers of well-spread cells with a characteristic striated pattern, while it did not react with myotubes containing organized myofibrils. On native ciliated cells as well as on Triton-extracted ciliated cortices from quail oviduct, this monoclonal antibody decorated the apical pole with a stronger labeling of the periphery of the apical area. Ultrastructural localization was attempted using the immunogold technique on the same preparation. Myosin was associated with a filamentous material present between striated rootlets and the proximal extremities of the basal bodies. No labeling of the basal body itself or of axoneme was observed.