Role of microRNA-21 in regulating 3T3-L1 adipocyte differentiation and adiponectin expression
Role of microRNA-21 in regulating 3T3-L1 adipocyte differentiation and adiponectin expression
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DOI：
10.1007/s11033-013-2603-6
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发表时间：
2013-08-01
影响因子：
2.8
通讯作者：
Ou, He-Sheng
中科院分区：
文献类型：
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作者：
Kang, Min;Yan, Li-Mei;Ou, He-Sheng
MicroRNA是一类高度保守的内源性小分子RNA，参与多种生命活动。本研究采用microRNA芯片技术筛选和鉴定脂肪相关microRNA，探讨microRNA对3 T3-L1脂肪细胞分化和脂联素表达的影响。构建表达上调明显的microRNA-21（miR-21）及其反义核酸（miR-21抑制剂）高表达质粒，转染3 T3-L1前脂肪细胞。观察miR-21对3 T3-L1脂肪细胞分化的影响，并分析脂联素和AP-1的蛋白和mRNA表达水平。结果表明，在3 T3-L1脂肪细胞分化过程中，microRNA的表达谱发生了显著变化。miR-21的表达明显上调。miR-21可显著促进脂肪细胞分化，增加脂联素mRNA和蛋白表达，降低AP-1蛋白水平。miR-21抑制剂可阻断miR-21的上述作用。AP-1的过表达可完全逆转miR-21对脂联素的刺激作用。miR-21通过抑制AP-1的表达在调节脂肪细胞分化和脂联素表达中起重要作用。
MicroRNAs are endogenous small RNAs with a high degree of conservation, participating in a variety of vital activities. In present study, to explore the effect of microRNAs on 3T3-L1 adipocyte differentiation and adiponectin expression, the adipo-related microRNAs were screened and identified by micorRNA microarray. The highly expression plasmid of microRNA-21 with obvious expression up-regulation (miR-21) and its anti-sense (miR-21 inhibitor) were constructed and transfected into 3T3-L1 preadipocytes. The effect of miR-21 on 3T3-L1 adipocyte differentiation was observed, and the protein and mRNA expression level of adiponectin and AP-1 were analyzed. Results showed that, the expression profiles of microRNAs significantly changed during 3T3-L1 adipocyte differentiation. The expression of miR-21 was obviously up-regulated. miR-21 could significantly promote adipocyte differentiation, increase adiponectin mRNA and protein expression, while decrease AP-1 protein level. Meanwhil, miR-21 inhibitor blocked the effects of miR-21 mentioned above. The overexpression of AP-1 could absolutely reverse the stimulatory effect of miR-21 on adiponectin. miR-21 plays an important role in regulating adipocyte differentiation and adiponectin expression by inhibiting AP-1 expression.