OCT4 Expression Enhances Features of Cancer Stem Cells in a Mouse Model of Breast Cancer.
OCT4 Expression Enhances Features of Cancer Stem Cells in a Mouse Model of Breast Cancer.
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DOI：
10.5625/lar.2011.27.2.147
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发表时间：
2011-06
影响因子：
2.9
通讯作者：
Nam JS
中科院分区：
文献类型：
--
作者：
Kim RJ;Nam JS
癌症干细胞（CSC）假说提出CSC是转移和疾病复发的原因。因此，靶向CSC有可能显著改善癌症患者的预后。OCT 4转录因子基因是在干细胞的自我更新和多能性中起关键作用的主基因。在这项研究中，我们将OCT 4报告载体引入4 T1小鼠乳腺癌细胞中，并分选OCT 4高和OCT 4低细胞群。然后，我们确定OCT 4表达是否与CSC的维持和扩增相关。我们发现，与OCT 4低4 T1细胞相比，OCT 4高4 T1细胞形成肿瘤球的能力增加，干细胞标志物如Sca-1、CD 133、CD 34和ALDH 1的表达增加。此外，OCT 4 high 4 T1细胞在体内具有更大的致瘤潜力。这些发现表明，OCT 4表达可能是干细胞特异性癌症治疗的有用靶点。
The cancer stem cell (CSC) hypothesis proposes that CSCs are responsible for metastasis and disease recurrence. Therefore, targeting CSCs has the potential to significantly improve outcomes for cancer patients. The OCT4 transcription factor gene is a master gene that plays a key role in the self-renewal and pluripotency of stem cells. In this study, we introduced an OCT4 reporting vector into 4T1 mouse breast cancer cells and sorted OCT4 high and OCT4 low cell populations. We then determined whether OCT4 expression is associated with maintenance and expansion of CSCs. We found that OCT4high 4T1 cells have an increased ability to form tumorsphere and a high expression of stem cell markers such as Sca-1, CD133, CD34, and ALDH1, when compared with OCT4low 4T1 cells. In addition, OCT4high 4T1 cells have greater tumorigenic potential in vivo. These findings suggest that OCT4 expression may be a useful target for stem cell-specific cancer therapy.