Seasonal variability in environmental parameters influence bacterial communities in mangrove sediments along an estuarine gradient
Seasonal variability in environmental parameters influence bacterial communities in mangrove sediments along an estuarine gradient
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DOI：
10.1016/j.ecss.2022.107791
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
S. O. Fernandes;Maria-Judith Gonsalves;D. Nazareth;Shu-Kuan Wong;Md. Nurul Haider;M. Ijichi;K. Kogure
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文献类型：
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作者：
S. O. Fernandes;Maria-Judith Gonsalves;D. Nazareth;Shu-Kuan Wong;Md. Nurul Haider;M. Ijichi;K. Kogure
红树林生态系统是不同微生物群落的储藏库，它们对环境特征的反应不同。因此，了解群落结构与相关的控制环境因素是很重要的。研究了印度西海岸曼多维河口上游、中游和河口3个红树林区域细菌群落向下的季节变化。对45个沉积物样品提取的基因组DNA进行了454焦磷酸测序分析。共有15008个可操作分类单元(OTU)被划分为54门127纲，再次确认红树林为生物多样性热点。甲型变形杆菌类成员占标签序列的35%，在所有地点都表现出显著的季节性变化(Kruskal-Wallis检验，p<0.05)。在研究地点还发现了其他种类丰富的类杆菌、藻类、放线杆菌、氯杆菌和酸杆菌等，表明它们参与了生物地球化学循环。环境变量的季节性波动影响了细菌群落。统计分析表明，在季风期间，由于NH4+在次表层沉积物中的积累，口部附近的细菌群落很大程度上受到孔隙水NH4+浓度的影响。在上游，季风过后，有机碳和二氧化氮−含量升高，导致细菌群落发生变化。
Mangrove ecosystems are repositories of divergent microbial communities which respond differently to environmental characteristics. Hence, it is important to understand the community structure with respect to pertinent controlling environmental factors. Seasonal down-core variations in bacterial communities were investigated at 3 mangrove sites at upstream, mid-stream and mouth region along the economically-important Mandovi estuary, west coast of India. Genomic DNA extracted from 45 sediment samples were analyzed by 454 pyrosequencing. A total of 15008 operational taxonomic units (OTUs) were assigned to 54 phyla and 127 classes re-affirming mangroves to be biodiversity hotspots. Members of class Alphaproteobacteria constituted up to 35% of the tag sequences and showed marked seasonal variability at all locations (Kruskal-Wallis test, p < 0.05). Occurrence of other abundant phyla like Bacteroidetes, Firmicutes, Actinobacteria, Chloroflexi and Acidobacteria at the study sites indicated their participation in biogeochemical cycling. Seasonal fluctuations in the environmental variables influenced the bacterial communities. Statistical analysis revealed that during the monsoon, bacterial communities proximal to the mouth region were largely influenced by pore water NH4+concentrations due to its accumulation in the sub-surface sediments. At the upstream site, elevated organic carbon and NO2−content during the post-monsoon caused variations in the bacterial community.